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Maxwell® HT 96 gDNA Blood Isolation System

Maxwell® HT 96 gDNA Blood Isolation System

High-Throughput DNA Extraction from Blood or Saliva in 24-or 96-Well Format

  • Robust, precipitation-free protocol, No chance of “lost pellets”.
  • High yields of pure DNA from pristine and challenged or hemolysed samples.
  • Pure gDNA ready for demanding applications; samples in solution; no resuspension required

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Description

Size

Catalog number selected: A2670

Catalog number selected: A2671

High-Throughput DNA Extraction from Blood or Saliva in 24-or 96-Well Format

 

 

Automation-Friendly DNA Purification: No Centrifugation, No Organic Solvents

The Maxwell® HT 96 gDNA Blood Isolation System provides a simple and reliable method for rapid isolation of genomic DNA in a multiwell format. gDNA may be purified from blood and Oragene®•Discover sample collection devices. The purified DNA can be used directly in PCR, microarrays and next-generation sequencing applications.

The use of paramagnetic particles for DNA capture eliminates the need for centrifugation or vacuum manifolds, making the system suitable for full automation. In addition, the system does not require an organic solvent, making it safe and convenient. DNA yields of up to 12µg are expected from input blood volumes of 350µl, depending on the white blood cell count of the sample. Saliva samples can have variable amounts of gDNA, and up to 18µg or more of DNA may be recovered from a 700µl Oragene® collection device sample.

Protocols

Complete Protocol

Maxwell® HT 96 gDNA Blood Isolation System Technical Manual

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Quick Protocols

Maxwell HT 96 gDNA Blood Isolation System Quick Protocol for Purification of Genomic DNA from Anticoagulated Whole Blood FB213

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Maxwell HT 96 gDNA Blood Isolation System Quick Protocol for Purification of Genomic DNA from Saliva Samples in Oragene®•Discover Devices FB214

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Specifications

What’s in the box?

Item Catlog number: A2670 Part # Size Concentration Available Separately
Binding Buffer (BBA) A174B 1 × 50ml View Product
Resin A175B 1 × 5.5ml View Product
Wash Buffer (WBA) A176B 1 × 85ml View Product
10mM EDTA (pH 8.0) A263A 1 × 10ml View Product
Proteinase K (PK) Solution A505C 1 × 5.5ml View Product
RNase A Solution A797C 1 × 1ml 4mg/ml View Product
25mM Tris-HCl (pH 8.0) A2641 1 × 60ml View Product
Cell Lysis Buffer (CLD) A173B 1 × 40ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

Item Catlog number: A2671 Part # Size Concentration Available Separately
Binding Buffer (BBA) A174C 1 × 200ml View Product
Resin A175B 4 × 5.5ml View Product
Wash Buffer (WBA) A176C 1 × 350ml View Product
10mM EDTA (pH 8.0) A263A 1 × 10ml View Product
25mM Tris-HCl (pH 8.0) A2641 1 × 60ml View Product
Proteinase K (PK) Solution A505D 1 × 23ml View Product
RNase A Solution A797E 1 × 5ml 4mg/ml View Product
Cell Lysis Buffer (CLD) A173C 1 × 160ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 6,855,499 and other patents.