Description
Hands-Free RNA Extraction in Under an Hour
The Maxwell® 16 LEV simplyRNA Kits are used with the Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor. This RNA purification procedure is a simple method with minimal lysate handling before automated purification. A low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. The kit provides the reagents for processing the samples and uses prefilled cartridges for purification. The Maxwell® 16 Instrument can process from 1 to 16 samples in under an hour.
The Maxwell® 16 LEV simplyRNA Kits currently run only on the Maxwell® 16 Instrument (AS2000) configured with the Maxwell® 16 High Strength Magnetic Rod and Plunger Bar Adaptor, as well as Firmware Version 4.9. For Maxwell® 16 Instruments (AS2000) purchased before May 2011, contact your Promega representative for information on how to configure your instrument for use with the simplyRNA kits.
RNA Yield (µg) from Tissues using the Maxwell® 16 LEV simplyRNA Tissue Kit
RNA Yield (µg) from Tissues using the Maxwell® 16 LEV simplyRNA Tissue Kit
Protocols
Complete Protocol
Quick Protocols
What’s in the box ?
Size: 48 preps
| Item - Catalog number: AS1310 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B | 1 × 900μl | |
| Cell Lysis Solution | A793A | 4 × 100ml | View Product |
| Maxwell® 16 LEV Cartridges (MCF) | AS131A | 48 × 1 each | |
| LEV Plungers | AS610A | 1 × 50/pk | View Product |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Proteinase K (PK) Solution | MC500C | 2 × 1ml | View Product |
| Lysis Buffer | MC501C | 1 × 20ml | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Homogenization Solution | Z305H | 1 × 30ml | |
| DNase I (lyophilized) | Z358A | 2 × 1 vial |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 48 preps
| Item - Catalog number: AS1270 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B | 1 × 900μl | |
| Maxwell® 16 LEV Cartridge (MCE) | AS128A | 48 × 1 each | |
| LEV Plungers | AS610A | 1 × 50/pk | View Product |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Lysis Buffer | MC501C | 1 × 20ml | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Homogenization Solution | Z305H | 1 × 30ml | |
| DNase I (lyophilized) | Z358A | 1 × 1 vial |
SDS
Download SDS
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
Size: 48 preps
| Item - Catalog number: AS1280 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B | 1 × 900μl | |
| Maxwell® 16 LEV Cartridge (MCE) | AS128A | 48 × 1 each | |
| LEV Plungers | AS610A | 1 × 50/pk | View Product |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Lysis Buffer | MC501C | 1 × 20ml | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Homogenization Solution | Z305H | 1 × 30ml | |
| DNase I (lyophilized) | Z358A | 2 × 1 vial |
SDS
Download SDS
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
Patents and Disclaimers
U.S. Pat. No. 6,855,499 and other patents.
