MagneSil® Total RNA mini-Isolation System

MagneSil® Total RNA mini-Isolation System

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Catalog number: Z3351

The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well procedure takes about 30 minutes to complete using a liquid-handling workstation.

Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.

  • Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
  • Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration.
  • DNase I treatment is included to remove genomic DNA contamination.


Complete Protocol

MagneSil® Total RNA mini-Isolation System Technical Bulletin

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What’s in the box?

Item - Catalog number: Z3351 Part # Size Available Separately
Nuclease-Free Water P1195 1 × 150ml View Product
RNA Lysis Buffer (RLA) Z305D 1 × 100ml View Product
DNase Stop Solution Z312D 1 × 26.5ml
Yellow Core Buffer Z317D 1 × 25ml
MnCl2, 0.09M Z318D 1 × 5ml
MagneSil® RNA Paramagnetic Particles Z336D 1 × 20ml
DNase I (lyophilized) Z358A 4 × 1 vial

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions