Loading...

HhaI

Description

10μ/μl

Catalog number selected: R6441

1,000u

Catalog number selected: R6441

Cut Site:

G  CGC
CGC  G

Notes:

  1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  2. HhaI is an isoschizomer of CfoI.

Incubation Conditions: Buffer C. 37°C.

Source: E. coli strain that carries the HhaI gene from Haemophilus haemolyticus (ATCC 10014).

Storage Buffer: 10mM Tris-HCl (pH 7.3), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
50–75% 75–100% 100% 50–75% 75–100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
215 375 18 17 26 31

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer C 10X Buffer R003A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
HhaI R644A 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions