Cut Site:

G  CG^▼C
C_▲GC  G

Notes:

1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
2. HhaI is an isoschizomer of CfoI.

Incubation Conditions: Buffer C. 37°C.

Source: E. coli strain that carries the HhaI gene from Haemophilus haemolyticus (ATCC 10014).

Storage Buffer: 10mM Tris-HCl (pH 7.3), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.