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DpnI

  • Capable of digesting DNA in 15 minutes or less

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Description

10μ/μl

Catalog number selected: R6231

200u

Catalog number selected: R6231

Cut Site:

GmeATC
CTmeAG

Usage Note: DpnI requires N6-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.

Incubation Conditions: Buffer B. 37°C.

Source: Diplococcus pneumoniae.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
50–75% 100% 75–100% 50–75% 100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
116 87 0 15 7 22

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer B 10X Buffer R002A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
DpnI R623A 1 × 200u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions