Description
Cut Site:
GmeAâ–ĽTC
CTâ–˛meAG
Usage Note:Â DpnI requires N6-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.
Incubation Conditions: Buffer B. 37°C.
Source: Diplococcus pneumoniae.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 50–75% | 100% | 75–100% | 50–75% | 100% | |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 116 | 87 | 0 | 15 | 7 | 22 |
Protocols
Complete Protocol
Â
 Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Buffer B 10X Buffer | R002A | 1 Ă— 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 Ă— 150ÎĽl | 10mg/ml | View Product |
| DpnI | R623A | 1 Ă— 200u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
