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BL21(DE3)pLysS Competent Cells

BL21(DE3)pLysS Competent Cells

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Description

Size

1ml (20 x 50µl)

Catalog number selected: L1195

1ml (5 x 200µl)

Catalog number selected:  L1191

BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site. BL21(DE3)pLysS is lysogenic for λ-DE3, which contains the T7 bacteriophage gene I, encoding T7 RNA polymerase under the control of the lac UV5 promoter. BL21(DE3)pLysS also contains a plasmid, pLysS, which carries the gene encoding T7 lysozyme. T7 lysozyme lowers the background expression level of target genes under the control of the T7 promoter but does not interfere with the level of expression achieved following induction by IPTG. For researchers performing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are available.

Genotype: F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr.

References

  1. Studier, F.W. and Moffatt, B.A. (1986) J. Mol. Biol. 189, 113–30.
  2. Davanloo, P. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 2035–9.

 

BL21(DE3) is an expression strain suitable for high level induction and expression of genes from any T7 promoter-based expression vector. T7 RNA polymerase expression is induced by the addition of 1mM IPTG to the culture.

Protocols

Complete Protocol

E. coli Competent Cells Technical Bulletin

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Quick Protocols

E Coli Competent Cells Quick Protocol FB035

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Specifications

What’s in the box?

You are viewing: L1191 Change Configuration

 

Item Part # Size
Competent Cells Control DNA L107A 1 × 30μl
BL21(DE3)pLysS Competent Cells, >106cfu/μg L119A 5 × 200μl

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: L1195 Change Configuration

 

Item Part # Size
Single-Use BL21(DE3)pLysS Competent Cells L119B 20 × 50μl

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions