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BclI

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Description

10μ/μl

Catalog number selected: R6651

100u

Catalog number selected: R6651

Cut Site:

TGATC  A
A  CTAGT

Notes:

  1. Sensitive to dam methylation.
  2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  3. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  4. Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.

Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)

Source: Bacillus caldolyticus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
10–25% 75–100% 100% 50–75% 10–25%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
8 5 0 0 0 0

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Buffer C 10X Buffer R003A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
BclI R665A 1 × 1,000u

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions