Cut Site:

T^▼GATC  A
A  CTAG_▲T

Notes:

1. Sensitive to dam methylation.
2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
3. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
4. Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.

Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)

Source: Bacillus caldolyticus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.