Description
Cut Site:
G GGCC▼C
C▲CCGG G
Note: Sensitive to overlapping dcm methylation.
Incubation Conditions: Buffer A. 37°C.
Source: Acetobacter pasteurianus.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | MULTI-CORE™ | |
| 100% | 50–75% | 50–75% | <10% | 75–100% | |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 1 | 12 | 0 | 0 | 0 | 0 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Buffer A 10X Buffer | R001A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 × 750μl | 10mg/ml | View Product |
| ApaI | R636A | 1 × 5,000u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
