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6 nt Indexing Kits

6 nt Indexing Kits

Description

The Lexogen i5 6 nt Dual Indexing Add-on Kit contains four perfectly balanced i5 Indices 5001-5004 (Cat. No. 047).

i5 (Index 2) indices can be introduced at the PCR step of CORALL Total RNA-Seq Kits (Cat. No. 095), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the four i5 indices can be combined with any of the 96 i7 indices (Index 1) (7001-7096) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 384 different barcoding options.

  • Kit size: 96 rxn of each i5 index
  • Compatibility information
    • Product: CORALL Total RNA-Seq and QuantSeq
    • Platform: Illumina

Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004) and Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096)

The Lexogen i5 6 nt Dual Indexing Add-on Kit (Cat. No. 047) contains perfectly balanced i5 indices. It is available in sets of 4 (5001-5004) and 96 (5001-5096) indices.

i5 (Index 2) indices can be introduced at the PCR step of CORALL (Cat. No. 095), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the i5 indices can be combined with any of the 96 i7 indices (Index 1) (7001-7096) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 9,216 different barcoding options.

  • Kit size: 96 rxn for each i5 index (5001-5004) and 1 rxn for each i5 index (5001-5096)
  • Compatibility information
    • Product: CORALL and QuantSeq
    • Platform: Illumina
  • Kit’s features
    • 96 i5 unique barcode combinations for fully-flexible dual indexing
    • 6 bp indices with perfect, uniform nucleotide and color balance
    • Hamming distance of 3 enables both error detection and error correction
    • Detect and quantify index hopping
    • Avoid cross-contamination and minimize index mis-assignment
    • Online index balance checker tool to assist with the choice of the optimal index combination for your experiment

pdf Lexogen i5 6 nt Dual Indexing Add-on Kits (5001-5096) Instruction Manual
pdf Lexogen i7 and i5 Index Sequences – update 05.05.2020

Product Items

Catalog Nr. Product Name
047.4×96 Lexogen i5 6 nt Dual Indexing Add-on Kit (5001-5004), 96 rxn/Index

Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096)

i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096)

Description

The Lexogen i5 6 nt Dual Indexing Add-on Kit contains a 96-well plate with 96 i5 indices (5000-5096) that can be used for any QuantSeq and CORALL for Illumina.

i5 (Index 2) indices can be introduced at the PCR step of CORALL Total RNA-Seq Kits (Cat. No. 095), QuantSeq 3’ mRNA-Seq Kits (Cat. No. 015 and 016), and QuantSeq-Flex RNA-Seq Kits (Cat. No. 033, 034, and 035). Each of the 96 unique i5 indices can be combined with any of the 96 unique i7 indices (Index 1) (7001-7096) included in Lexogen’s RNA-Seq Kits for Illumina, enabling up to 9,216 different barcoding options.

  • Kit features:
    • 96 i5 unique barcode combinations for fully-flexible dual indexing
    • 6 bp indices with perfect, uniform nucleotide and color balance
    • Hamming distance of 3 enables both error detection and error correction
    • Detect and quantify index hopping
    • Avoid cross-contamination and minimize index mis-assignment
    • Online index balance checker tool to assist with the choice of the optimal index combination for your experiment
  • Kit size: 96 rxn (1 rxn/index)
  • Compatibility information
    • Product: CORALL Total RNA-Seq and QuantSeq
    • Platform: Illumina

Product Items

Catalog Nr. Product Name
047.96 Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (5001-5096), 1rx/Index

Lexogen i7 6 nt Index Set (7001-7096)

Lexogen i7 6 nt Index Set (7001-7096)


Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any QuantSeq or CORALL kit for Illumina.

NOTE: As of February 2019 barcode plates have been renamed to Lexogen i7 6 nt Index Set (7001-7096).

pdf Lexogen i7 and i5 Index Sequences – update 05.05.2020

Description

Includes a 96-well plate with 96 i7 indices (7000-7096) that can be used for any CORALL and QuantSeq kit for Illumina.

NOTE: As of February 2019 barcode plates have been renamed to Lexogen i7 6 nt Index Set (7001-7096).

Product Items

Catalog Nr. Product Name
044.96 Lexogen i7 6 nt Index Set (7001-7096), 1 rxn/Index