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Nucleic Acid Purification and Quantitation

Nucleic Acid Purification and Quantitation

Nucleic Acid Purification and Quantitation

In today’s world of nucleic acid analysis by real-time PCR and next-generation sequencing, it’s crucial to purify high-quality nucleic acids from your samples. Once you accomplish that, virtually every downstream assay requires that you quantitate your DNA or RNA before you begin. Understanding how these systems work and then choosing the best chemistry for your application is essential.

In today’s world of nucleic acid analysis by real-time PCR and next-generation sequencing, it’s crucial to purify high-quality nucleic acids from your samples. Once you accomplish that, virtually every downstream assay requires that you quantitate your DNA or RNA before you begin. Understanding how these systems work and then choosing the best chemistry for your application is essential.

Molecular Biology Lab Guide

From basic DNA purification to the most complicated qPCR experiment, the Molecular Biology Lab Guide can help you make every experiment successful.

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Nucleic Acid Quantitation

Molecular Biology Rule #1: Never skip quantitating your nucleic acids. This important step helps you make sure you’re ready for your next application. Maybe you’ll get lucky, but you risk having to start over when you skip quantitation. No one wants to do that.