Lumit™ Cytokine Assays: Interpolating Data with the GloMax® Discover
Publication Date: June 2021, tpub_222
Lumit™ Cytokine Immunoassays are rapid and convenient assays to quantify cytokine release from cultured cells. These add-and-read assays are based on NanoBiT® technology using antibodies labeled with SmBiT and LgBiT, the complementary parts of the NanoBiT® luciferase. SmBiT and LgBiT-labeled antibodies directed against a specific cytokine generate light when bound to the cytokine followed by addition of the detection reagent that includes luciferase substrate. Light is generated proportional to the amount of cytokine released. Standard curves of known concentrations of cytokine enable the conversion of the Relative Light Unit (RLU) readout to concentrations of cytokine. There are several ways to interpolate data from a standard curve, but the variety of method choices can lead to confusion. Furthermore, historical methods may not always be the best choice for a new assay.
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