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SpeI

  • Capable of digesting DNA in 15 minutes or less
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications

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Description

10μ/μl

Catalog number selected: R6591

200u

Catalog number selected: R6591

1,000u

Catalog number selected: R6595

Cut Site:

ACTAG   T
T   GATCA

Usage Note: Ends generated with SpeI can be directly ligated to ends generated by NheI or XbaI. None of these recognition sites are regenerated in the ligation product.

Incubation Conditions: Buffer B. 37°C.

Source: Sphaerotilus natans ATCC 13923.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
75–100% 100% 75–100% 75–100% 100%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
0 3 0 0 0 0

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

You are viewing: R6595 Change Configuration
Item Part # Size Concentration Available Separately
Buffer B 10X Buffer R002A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
SpeI R659A 1 × 200u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6591 Change Configuration
Item Part # Size Concentration Available Separately
Buffer B 10X Buffer R002A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
SpeI R659D 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions