Description
The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.
Applications of the Riboprobe® Systems include:
- Generation of high-specific-activity RNA probes.
- Generation of microgram quantities of RNA transcripts.
- Generation of RNA processing substrates.
- Generation of antisense RNA.
Protocols
What’s in the box?
Item / Catalog number: P1460 | Part # | Size | Concentration | Available Separately |
RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product | |
Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
rATP | P113A | 2 × 50μl | 10mM | View Product |
rCTP | P114A | 2 × 50μl | 10mM | View Product |
rGTP | P115A | 2 × 50μl | 10mM | View Product |
rUTP | P116A | 2 × 50μl | 10mM | View Product |
DTT | P117A | 2 × 100μl | 100mM | View Product |
Transcription Optimized 5X Buffer | P118B | 1 × 500μl | View Product | |
Nuclease-Free Water | P119A | 2 × 1,250μl | View Product | |
SP6 RNA Polymerase | P108E | 1 × 500u | View Product | |
T7 RNA Polymerase | P207D | 1 × 500u | View Product | |
pGEM® Express Positive Control Template | P256A | 1 × 5μg | View Product |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Item / Catalog number: P1450 | Part # | Size | Concentration | Available Separately |
RQ1 RNase-Free DNase | M610C | 1 × 110u | View Product | |
Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
rATP | P113A | 2 × 50μl | 10mM | View Product |
rCTP | P114A | 2 × 50μl | 10mM | View Product |
rGTP | P115A | 2 × 50μl | 10mM | View Product |
rUTP | P116A | 2 × 50μl | 10mM | View Product |
DTT | P117A | 2 × 100μl | 100mM | View Product |
Transcription Optimized 5X Buffer | P118B | 1 × 500μl | View Product | |
Nuclease-Free Water | P119A | 2 × 1,250μl | View Product | |
T7 RNA Polymerase | P207D | 1 × 500u | View Product | |
T3 RNA Polymerase | P208A | 1 × 500u | View Product | |
pGEM® Express Positive Control Template | P256A | 1 × 5μg | View Product |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions