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Riboprobe® Combination Systems

Riboprobe® Combination Systems

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Description

Catalog number :  P1460

Catalog number : P1450

The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.

Applications of the Riboprobe® Systems include:

  • Generation of high-specific-activity RNA probes.
  • Generation of microgram quantities of RNA transcripts.
  • Generation of RNA processing substrates.
  • Generation of antisense RNA.

Protocols

Complete Protocol

Riboprobe® In Vitro Transcription Systems Technical Manual

Specifications

What’s in the box?

Item / Catalog number: P1460 Part # Size Concentration Available Separately
RQ1 RNase-Free DNase M610C 1 × 110u View Product
Recombinant RNasin® Ribonuclease Inhibitor N251A 1 × 2,500u 40u/μl View Product
rATP P113A 2 × 50μl 10mM View Product
rCTP P114A 2 × 50μl 10mM View Product
rGTP P115A 2 × 50μl 10mM View Product
rUTP P116A 2 × 50μl 10mM View Product
DTT P117A 2 × 100μl 100mM View Product
Transcription Optimized 5X Buffer P118B 1 × 500μl View Product
Nuclease-Free Water P119A 2 × 1,250μl View Product
SP6 RNA Polymerase P108E 1 × 500u View Product
T7 RNA Polymerase P207D 1 × 500u View Product
pGEM® Express Positive Control Template P256A 1 × 5μg View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

What’s in the box?

Item / Catalog number: P1450 Part # Size Concentration Available Separately
RQ1 RNase-Free DNase M610C 1 × 110u View Product
Recombinant RNasin® Ribonuclease Inhibitor N251A 1 × 2,500u 40u/μl View Product
rATP P113A 2 × 50μl 10mM View Product
rCTP P114A 2 × 50μl 10mM View Product
rGTP P115A 2 × 50μl 10mM View Product
rUTP P116A 2 × 50μl 10mM View Product
DTT P117A 2 × 100μl 100mM View Product
Transcription Optimized 5X Buffer P118B 1 × 500μl View Product
Nuclease-Free Water P119A 2 × 1,250μl View Product
T7 RNA Polymerase P207D 1 × 500u View Product
T3 RNA Polymerase P208A 1 × 500u View Product
pGEM® Express Positive Control Template P256A 1 × 5μg View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB