Description
Size: 10 preps
Catalog number: Z6010
Size: 50 preps
Catalog number: Z6011
Size: 250 preps
Catalog number: Z6012
Size: 10 preps
Catalog number: Z6110
Size: 50 preps
Catalog number: Z6111
Size: 250 preps
Catalog number: Z6112
Recover Concentrated, Pure RNA from Precious Cell and Tissue Samples
Precious samples, sensitive assays, low copy messages – the effectiveness of RNA purification sets the stage for downstream results.
The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl).
Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.
- Small sample size and low elution volume allows conservation of precious samples.
- Maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets.
- Usable, concentrated RNA is eluted in as little as 7µl. No need to further concentrate samples.
- Rapid protocol and provided DNase reagents streamline laboratory processes.
- Purified RNA is suitable for many common downstream assays.
Higher RNA Yield
The data to the right show that the ReliaPrep™ Cell RNA Miniprep System efficiently isolates RNA from 100 to 1 x 106 HeLa cells with a higher RNA yield than the leading competitor. Below, the RNA yield from 100,000 cells of 4 different cell lines. More data on performance of the ReliaPrep™ Systems is available in this Application Note.
Yield and Purity of RNA from Tissues
This data shows typical RNA yields from fibrous and nonfibrous mouse tissues using the ReliaPrep™ RNA Tissue Miniprep System. RNA was isolated from 10mg samples of various mouse tissues. Yield and purity were determined by absorbance spectroscopy using a Nanodrop® 1000 instrument.
More data illustrating use of the ReliaPrep™ RNA Tissue Miniprep System is available in this Application Note.
Protocols
Quick Protocols
What’s in the box?
Size: 10 preps
Item - Catalog number: Z6010 | Part # | Size | Available Separately |
Collection Tubes | A130C | 2 × 10/pk | |
ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
1-Thioglycerol | A208A | 1 × 75μl | |
Column Wash Solution (CWE) | A254A | 1 × 1ml | |
Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
BL Buffer | Z103A | 2 × 3.25ml | |
Elution Tubes | Z200B | 1 × 25/pk | |
RNA Wash Solution (RWA) | Z309B | 1 × 11.8ml | View Product |
Yellow Core Buffer | Z317B | 1 × 300μl | |
MnCl2, 0.09M | Z318A | 1 × 250μl | |
DNase I (lyophilized) | Z358B | 1 × 1 vial |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 50 preps
Item - Catalog number: Z6011 | Part # | Size | Available Separately |
Collection Tubes | A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns | A207B | 1 × 50/pack | |
1-Thioglycerol | A208B | 1 × 900μl | |
Column Wash Solution (CWE) | A254B | 1 × 5ml | |
Nuclease-Free Water | P119E | 1 × 13ml | View Product |
BL Buffer | Z103B | 1 × 32.5ml | |
Elution Tubes | Z200C | 1 × 50/pk | |
RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
Yellow Core Buffer | Z317A | 1 × 2.5ml | |
MnCl2, 0.09M | Z318A | 1 × 250μl | |
DNase I (lyophilized) | Z358A | 1 × 1 vial |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 250 preps
Item - Catalog number: Z6012 | Part # | Size | Available Separately |
Collection Tubes | A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns | A207B | 5 × 50/pack | |
1-Thioglycerol | A208B | 2 × 900μl | |
Column Wash Solution (CWE) | A254C | 1 × 24ml | |
Nuclease-Free Water | P119E | 1 × 13ml | View Product |
BL Buffer | Z103C | 1 × 150ml | |
Elution Tubes | Z200C | 5 × 50/pk | |
RNA Wash Solution (RWA) | Z309D | 1 × 206ml | View Product |
Yellow Core Buffer | Z317C | 1 × 11ml | |
MnCl2, 0.09M | Z318B | 2 × 750μl | |
DNase I (lyophilized) | Z358A | 3 × 1 vial |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 10 preps
Item - Catalog number: Z6110 | Part # | Size | Available Separately |
Collection Tubes | A130C | 2 × 10/pk | |
ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
1-Thioglycerol | A208A | 2 × 75μl | |
Column Wash Solution (CWE) | A254A | 1 × 1ml | |
Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
Lysis Buffer (LBA) | Z106A | 1 × 6ml | |
Elution Tubes | Z200B | 1 × 25/pk | |
RNA Wash Solution (RWA) | Z309B | 1 × 11.8ml | View Product |
Yellow Core Buffer | Z317B | 1 × 300μl | |
MnCl2, 0.09M | Z318A | 1 × 250μl | |
DNase I (lyophilized) | Z358B | 1 × 1 vial | |
RNA Dilution Buffer (RDB) | Z372C | 1 × 9ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 50 preps
Item - Catalog number: Z6111 | Part # | Size | Available Separately |
Collection Tubes | A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns | A207B | 1 × 50/pack | |
1-Thioglycerol | A208B | 2 × 900μl | |
Column Wash Solution (CWE) | A254B | 1 × 5ml | |
Nuclease-Free Water | P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) | Z106B | 1 × 30ml | |
Elution Tubes | Z200C | 1 × 50/pk | |
RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
Yellow Core Buffer | Z317A | 1 × 2.5ml | |
MnCl2, 0.09M | Z318A | 1 × 250μl | |
DNase I (lyophilized) | Z358A | 1 × 1 vial | |
RNA Dilution Buffer (RDB) | Z372A | 1 × 44ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 250 preps
Item - Catalog number: Z6112 | Part # | Size | Available Separately |
Collection Tubes | A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns | A207B | 5 × 50/pack | |
1-Thioglycerol | A208B | 4 × 900μl | |
Column Wash Solution (CWE) | A254C | 1 × 24ml | |
Nuclease-Free Water | P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) | Z106C | 1 × 150ml | |
Elution Tubes | Z200C | 5 × 50/pk | |
RNA Wash Solution (RWA) | Z309D | 1 × 206ml | View Product |
Yellow Core Buffer | Z317C | 1 × 11ml | |
MnCl2, 0.09M | Z318B | 2 × 750μl | |
DNase I (lyophilized) | Z358A | 3 × 1 vial | |
RNA Dilution Buffer (RDB) | Z372B | 1 × 220ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.