Scalable, Walkaway DNA Purification from Large-Volume Blood Samples
The ReliaPrep™ Large Volume HT gDNA Isolation System is used with the HSM 2.0 Instrument (Cat.# A2715) to provide a scalable, efficient method for isolating genomic DNA (gDNA) from 1–10ml blood samples. The precipitation-free ReliaPrep™ DNA purification chemistry eliminates the tedious centrifugation steps and hazardous chemicals associated with precipitation-based methods, reducing purification failures. Each kit provides enough reagents to process up to 96 × 10ml whole blood samples.
The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of 1–10ml whole blood, regardless of sample storage or shipping conditions.
Genomic DNA yields from 10ml normal whole blood samples are typically 200–400µg (depending on white blood cell count) in a final eluted volume of 1ml. Recovered DNA exhibits good purity with A260/A280 ratios greater than 1.7 and A260/A230 ratios between 1.8 and 2.2.
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Expedite Sample Processing with the HSM 2.0 Heater Shaker Magnet
The HSM 2.0 Instrument heats, shakes and magnetizes sample tubes in one position throughout the extraction process from lysis to elution, eliminating the need to move samples on the robot deck and reducing instrument failures.
For low-throughput isolation of gDNA from up to 32 samples at one time, the HSM 2.0 instrument can be used in a semi-automated mode, where the user performs the pipetting functions. The HSM has software that controls the instrument and directs the user through the purification protocol.
No Manual Sample Transfer Once Instrument Is Loaded
Simply place the primary blood sample tubes on the instrument or transfer samples to processing tubes, follow the setup instructions and start the protocol. Purified DNA is ready in ≤4.5 hours.
Process Multiple Sample Types
|Human Whole Blood||Yes||No||Isolating gDNA from Blood: Simplified gDNA Isolation from Human Blood using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN186, Promega Corporation|
|Buffy Coat||Yes||No||Isolating gDNA from Buffy Coat Samples: Semi-Automated or Fully Automated Isolation of gDNA from Buffy Coat Samples Using the ReliaPrep™ Large Volume HT gDNA Isolation System. Application Note AN185, Promega Corporation|
What’s in the box?
|Item/ Catalog number selected: A2751||Part #||Size||Available Separately|
|Alkaline Protease (APA)||A1721||1 × 130ml||View Product|
|Cell Lysis Buffer (CLD)||A1731||1 × 1,400ml||View Product|
|Binding Buffer (BBA)||A1741||1 × 1,600ml||View Product|
|ReliaPrep™ Resin||A1752||1 × 115ml||View Product|
|Prepared Wash Buffer (WBC)||A2681||1 × 3,500ml||View Product|
|Proteinase K (PK) Solution||A5051||1 × 23ml||View Product|
|Nuclease-Free Water||P1195||4 × 150ml||View Product|
For Research Use Only. Not for Use in Diagnostic Procedures.
Patents and Disclaimers
U.S. Pat. No. 6,855,499 and other patents.