Description
Concentrate DNA from large volumes to ≤15µl
Prepare DNA samples for downstream applications
The ReliaPrep™ DNA Clean-Up and Concentration System is designed to quickly concentrate and purify dilute DNA solutions, extract and purify DNA fragments of 100bp–10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) buffer, or to purify products directly from a PCR amplification. This membrane-based system can bind up to 60µg of DNA and concentrate as much as 300µl of dilute DNA, recovering isolated DNA fragments or PCR products in as little as 10 minutes, depending on the number of samples processed and the protocol used.
A single reagent stream is used for all three procedures, making the system both fast and easy. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, next-generation sequencing or in vitro transcription/translation.
The ReliaPrep™ DNA Clean-Up and Concentration System can be used with linear DNA fragments, supercoiled plasmid DNA or single-stranded linear or circular DNA.
DNA (Cat.# G2101) was diluted in triplicate into a final 100µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Average yield and purity was 90.8%. Samples were further analyzed on an Agilent Bioanalyzer® 2100 using a DNA 1000 chip and protocol.
Protocols
What’s in the box ?
| Item - Catalog number: A2891 | Part # | Size | Available Separately |
| Collection Tubes | A130C | 1 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| Column Wash Solution (CWE) | A254A | 1 × 1ml | |
| Buffer B (BWB) | A288A | 1 × 11.8ml | |
| Membrane Binding Solution | A930A | 1 × 4ml | View Product |
| Elution Tubes | Z200A | 2 × 5/pk | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
without Vacuum Adapters
| Item - Catalog number: A2892 | Part # | Size | Available Separately |
| Column Wash Solution (CWE) | A254B | 1 × 5ml | |
| Buffer B (BWB) | A288A | 1 × 11.8ml | |
| Membrane Binding Solution | A930B | 1 × 20ml | View Product |
| Elution Tubes | Z200B | 2 × 25/pk | |
| Collection Tubes | A130A | 1 × 50/pk | |
| ReliaPrep™ Minicolumns | A207B | 1 × 50/pack | |
| Nuclease-Free Water | P119A | 1 × 1,250μl | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box ?
| Item - Catalog number: A2893 | Part # | Size | Available Separately |
| Column Wash Solution (CWE) | A254C | 1 × 24ml | |
| Buffer B (BWB) | A288B | 1 × 58.8ml | |
| Membrane Binding Solution | A930B | 2 × 20ml | View Product |
| Elution Tubes | Z200C | 5 × 50/pk | |
| ReliaPrep™ Minicolumns | A207B | 5 × 50/pack | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Collection Tubes | A130B | 1 × 250/pk |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
