Description
Sensitive Quantitation of Double-Stranded DNA in Solution
Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes
The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important. This dye shows minimal binding to single stranded DNA (ssDNA), RNA, protein and interfering compounds, allowing specific quantitation of dsDNA.
Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems
The Quantifluor dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 10pg/µl vs. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume).
| Sample* | Assay | |
| Quantifluor® dsDNA System | 0.01–200ng/µl | 0.01–200ng |
| Quant-iT™ PicoGreen | 0.02–200ng/µl | 0.02–200ng |
| Qubit® dsDNA HS | 0.2–100ng/µl | 0.2–100ng |
| NanoDrop® 2000 Spectrophotometer | 2–12,000ng/µl | 2–12,000ng |
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
Better Sensitivity than Quant-iT™ PicoGreen®, Equivalent Linearity
The QuantiFluor® dsDNA System detects as little as 50pg/ml dsDNA in a 96-well microplate assay (1µl of a 10ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.
How do fluorescent dyes compare to other quantitation methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Quick Protocols
What’s in the box?
| Item Catlog number: E2670 | Part # | Size |
| QuantiFluor® dsDNA Dye | E258A | 1 × 1ml |
| Lambda DNA Standard | E259A | 1 × 100μg |
| 20X TE Buffer (pH 7.5) | E260A | 1 × 25ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item Catlog number: E2671 | Part # | Size |
| QuantiFluor™ dsDNA Dye, 200X | E258B | 1 × 100μl |
| Lambda DNA Standard | E259B | 1 × 10μg |
| 20X TE Buffer (pH 7.5) | E260B | 1 × 5ml |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
