Description
Size:
5ml : Catalog number selected: A6001
25ml: Catalog number selected: A6002
Size:
5ml : Catalog number selected: A6020
Size:
5ml : Catalog number selected: A6010
Simple, Sensitive, Dye-Based qPCR and RT-qPCR
The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.
Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.
Choose qPCR or RT-qPCR Master Mix Formats
Sensitivity and Specificity for qPCR
The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.
Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:
- Sensitive detection for earlier quantitation of low- and high-copy targets.
- Room-temperature setup,making it suitable for automation and high-throughput detection.
- Compatibility with both fast and standard qPCR cycling methods.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6001 | 5ml | 1,000 reactions | 500 reactions |
| A6002 | 25ml | 5,000 reactions | 2,500 reactions |
qPCR with single-copy detection. The GAPDH gene was amplified and detected from 10-fold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (Slope=-3.2; R2=0.995). Kit yield and purity were measured by absorbance spectroscopy.
Reverse Transcription and qPCR in One Reaction
The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.
Other features provided by the 1-Step System include:
- Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
- Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6020 | 5ml | 1,000 reactions | 500 reactions |
Linear dynamic range from 100ng down to 10fg of human RNA template. Seven 10-fold dilutions of human total RNA was run using 200nM of each GAPDH transcript specific primer. The amplification curve shows 97% efficiency and an r2=0.999.
Two-Step Reverse Transcription and qPCR
The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.
The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
| Size | Cat.# | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6010 | 5ml | 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions | 50 × 20µl RT reactions and 500 × 20µl qPCR reactions |
Extended linear range. Dilutions of human reference total RNA were amplified with GoTaq® 2-Step RT-qPCR System looking at 5.8S expression. GoTaq® 2-Step system demonstrates a linear dynamic range over 9 orders of magnitude with a high fluorescent signal. NTC reactions were all blank.
Suitable for Different Organisms and Sample Types
DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.
Organism Types:
- Animal
- Bacteria
- Fungi
- Human
- Parasites
- Viruses
- Yeast
Sample Types:
- Blood
- Cells
- Feces
- FFPE
- Food
- Human Cerebrospinal Fluid (CSF)
- Plants
- Plasma
- Saliva
- Serum
- Sputum
- Swabs
- Tissue
- Urine
Protocols
Video Protocols
What’s in the box?
| Item/ size 5ml : Catalog number selected: A6001 | Part # | Size | Available Separately | Concentration |
| GoTaq® qPCR Master Mix, 2X | A600A | 5 × 1ml | ||
| CXR Reference Dye | C541A | 1 × 100μl | View Product | |
| Nuclease-Free Water | P119E | 2 × 13ml | View Product | |
| Item/ size 25ml: Catalog number selected: A6002 | Part # | Size | Available Separately | Concentration |
| GoTaq® qPCR Master Mix, 2X | A600A | 25 × 1ml | ||
| CXR Reference Dye | C541A | 5 × 100μl | View Product | 30μM |
| Nuclease-Free Water | P119E | 10 × 13ml | View Product |
SDS For A6001
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SDS For A6002
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item/ size 5ml : Catalog number selected: A6020 | Part # | Size | Concentration | Available Separately |
| MgCl2 | A351B | 1 × 750μl | 25mM | View Product |
| GoTaq® qPCR Master Mix, 2X | A600A | 5 × 1ml | ||
| Nuclease-Free Water | P119E | 2 × 13ml | View Product | |
| CXR Reference Dye | C541B | 1 × 200μl | 30μM | View Product |
| GoScript™ RT Mix for 1-Step RT-qPCR | M700A | 1 × 225μl | 50X |
SDS For A6020
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item/ size 5ml : Catalog number selected: A6010 | Part # | Size | Concentration | Available Separately |
| MgCl2 | A351B | 1 × 750μl | 25mM | View Product |
| GoScript™ 5X Reaction Buffer | A500B | 1 × 300μl | View Product | |
| GoScript™ Reverse Transcriptase | A501B | 1 × 50μl | ||
| GoTaq® qPCR Master Mix, 2X | A600A | 5 × 1ml | ||
| Oligo(dT)15 Primer | C110B | 1 × 50μg | 500μg/ml | View Product |
| PCR Nucleotide Mix | C114G | 1 × 200μl | 10mM | View Product |
| Random Primers | C118B | 1 × 50μg | View Product | |
| CXR Reference Dye | C541A | 1 × 100μl | View Product | |
| Recombinant RNasin® Ribonuclease Inhibitor | N251A | 1 × 2,500u | 40u/μl | View Product |
| Nuclease-Free Water | P119E | 2 × 13ml | View Product |
SDS For A6010
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
