Description
The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.
PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.
Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.
Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.
Protocols
What’s in the box?
| Item - Catalog number: A2492 | Part # | Size | Available Separately |
| Neutralization Solution (NSB) | A148H | 1 × 130ml | View Product |
| PureYield™ Binding Columns | A245A | 5 × 5 each | |
| PureYield™ Clearing Columns | A246A | 5 × 5 each | |
| Endotoxin Removal Wash | A251C | 1 × 85.3ml | |
| Column Wash | A252C | 1 × 210ml | |
| Cell Resuspension Solution (CRA) | A711F | 1 × 75ml | View Product |
| Cell Lysis Solution (CLA) | A712F | 1 × 75ml | View Product |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item - Catalog number: A2495 | Part # | Size | Available Separately |
| Neutralization Solution (NSB) | A1485 | 1 × 500ml | |
| PureYield™ Binding Columns | A245A | 20 × 5 each | |
| PureYield™ Clearing Columns | A246A | 20 × 5 each | |
| Endotoxin Removal Wash | A251B | 1 × 315ml | |
| Column Wash | A252B | 2 × 381ml | |
| Cell Resuspension Solution (CRA) | A7115 | 1 × 315ml | |
| Cell Lysis Solution (CLA) | A7125 | 1 × 315ml | |
| Nuclease-Free Water | P119C | 4 × 25ml | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
| Item - Catalog number: A2496 | Part # | Size | Available Separately |
| Neutralization Solution (NSB) | A1485 | 3 × 500ml | |
| PureYield™ Binding Columns | A245E | 3 × 100 each | |
| PureYield™ Clearing Columns | A246E | 3 × 100 each | |
| Endotoxin Removal Wash | A251B | 3 × 315ml | |
| Column Wash | A252B | 6 × 381ml | |
| Cell Resuspension Solution (CRA) | A7115 | 3 × 315ml | |
| Cell Lysis Solution (CLA) | A7125 | 3 × 315ml | |
| Nuclease-Free Water | P1195 | 2 × 150ml | View Product |
SDS
Download SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
