Description
Cut Site:
C TGCA▼G
G▲ACGT C
Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Ends generated by PstI can be directly ligated to ends generated by NsiI. Neither restriction site is regenerated in the ligation product.
Incubation Conditions: Buffer H. 37°C.
Source: Recombinant E. coli strain.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | H | MULTI-CORE™ |
| 10–25% | 50–75% | 50–75% | 50–75% | 100% | 25–50% |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 28 | 30 | 1 | 1 | 1 | 1 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6111 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer H 10X Buffer | R008A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| PstI | R611A | 1 × 3,000u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6115 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer H 10X Buffer | R008A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 × 750μl | 10mg/ml | View Product |
| PstI | R611B | 1 × 15,000u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
