Description
Size: 200 reactions
Catalog number: DC1001
Size: 800 reactions
Catalog number: DC1000
The Power to See It All in a Single Assay
The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors and a melt curve function to confirm that the correct product was amplified. This sensitive multiplex kit provides consistent DNA detection down to 6.4pg of total DNA. PCR setup is performed at room temperature and is compatible with automated platforms.
The Plexor® HY System is compatible with automated platforms and optimized for use on the Applied Biosystems 7500 and 7500 FAST real-time PCR systems and Stratagene Mx3005P® and Mx3000P® qPCR systems
The Plexor® Analysis Software is available for free download. The unique functions of this software allow you to quickly and easily review data and create reports. Replicate samples are automatically averaged, template amounts are calculated and the necessary volume of DNA is displayed for your optimized STR amplification conditions.
If There is DNA to Detect, the Plexor® HY System Will Show It
The system works by measuring a reduction in fluorescent signal during amplification. Amplification of each target uses only two primers, one of which contains both a fluorescent tag and a modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction of fluorescent signal. After PCR, a melt analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting.
Want to Automate?
Save time and improve precision by automating your DNA quantification set up. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplifications set up.
Automate your DNA Quantification Set up
Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.
Hardware
| Product | Cat.# |
|---|---|
|
Four-Position Tube Holder
|
V1601
|
|
Plate Clamp 96
|
V8251
|
|
Plate Stand
|
V8261
|
Protocols
Complete Protocol
Identity Automation™ qPCR Setup Protocol for the PowerQuant® and Plexor® HY Systems on the Tecan Freedom EVO® Workstation
Identity Automation™ Plexor® HY System Setup for the Beckman Coulter Biomek® 4000
Plexor® HY System for the Stratagene Mx3000P® and Mx3005P™ Quantitative PCR Systems Technical Manual
Quick Protocols
What’s in the box?
Size: 200 reactions
| Item - Catalog number: DC1001 | Part # | Size | Concentration | Available Separately |
| Plexor® HY Male Genomic DNA Standard | DD149A | 1 × 150μl | 50ng/μl | |
| Plexor® HY 20X Primer/IPC Mix | DK716A | 1 × 200μl | ||
| Plexor® HY 2X Master Mix | DP120A | 2 × 1ml | ||
| Water, Amplification Grade | DW099A | 2 × 1,250μl | View Product |
What’s in the box?
Size: 800 reactions
| Item - Catalog number: DC1000 | Part # | Size | Concentration | Available Separately |
| Plexor® HY Male Genomic DNA Standard | DD149A | 3 × 150μl | 50ng/μl | |
| Plexor® HY 20X Primer/IPC Mix | DK716A | 4 × 200μl | ||
| Plexor® HY 2X Master Mix | DP120A | 8 × 1ml | ||
| Water, Amplification Grade | DW099A | 5 × 1,250μl | View Product |
What’s in the box?
Size: 1 each
| Item - Catalog number: DC1500 | Part # | Size |
| Plexor® Concentrated Fluorescein Calibrator | DS101A | 1 × 50μl |
| Plexor® Concentrated CAL Fluor® Orange 560 Calibrator | DS102A | 1 × 50μl |
| Plexor® Concentrated CAL Fluor® Red 610 Calibrator | DS103A | 1 × 50μl |
| Plexor® Concentrated IC5 Calibrator | DS104A | 1 × 50μl |
| Plexor® 1X Calibration Buffer | DS105A | 1 × 11ml |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.
The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.
This product is sold under licensing arrangements with Stratagene. The purchase price of this product includes limited, nontransferable rights under U.S. Pat. Nos. 5,449,603, 5,605,824, 5,646,019 and 5,773,257 owned by Stratagene to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the “Forensic and Genetic Identity Applications Field”). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the Business Development Department, Stratagene California, 11011 North Torrey Pines Road, La Jolla, CA 92037.
This product is sold under licensing arrangements with the USB Corporation. The purchase price of this product includes limited, nontransferable rights under U.S. Patent Application Serial Number 11/171,008 owned by the USB Corporation to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the “Forensic and Genetic Identity Applications Field”). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the USB Corporation, 26111 Miles Road, Cleveland, OH 44128.
