Description
Choose a protein fusion
The pH6HTN and pH6HTC His6HaloTag® T7 Vectors are used for inducible expression of polyhistidine-HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied as multiple cloning site (MCS) vectors for traditional cloning.
pH6HTN His6HaloTag® T7 Vector (Cat.# G7971) is designed for protein expression with an N-terminal His6-HaloTag® dual tag; pH6HTC His6HaloTag® T7 Vector (Cat.# G8031) is designed for protein expression with a C-terminal His6-HaloTag® dual tag. By coupling the protein solubility and labeling benefits of HaloTag® technology with the reusability and the throughput of Ni-affinity technology, the dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag® covalent binding with HaloTag® ligands.
pH6HTC His6HaloTag® T7 Vector GenBank® Accession Number JN874647 and vector sequence text file.
pH6HTN His6HaloTag® T7 Vector GenBank® Accession Number JN874646 and vector sequence text file.
HaloTag® Technology improves purification of difficult proteins.
HaloTag® Technology improves purification of difficult proteins.
Protocols
Complete Protocol
pH6HTN His6HaloTag® T7 Vector Product Information
What’s in the box?
Size: 20µg
| Item/Catalog number: G8031 | Part # | Size |
|---|---|---|
| pH6HTC His6HaloTag® T7 Vector | G803A | 1 × 20μg |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Size: 20µg
| Item / Catlog no: G7971 | Part # | Size |
| pH6HTN His6HaloTag® T7 Vector | G797A | 1 × 20μg |
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.
