Loading...

pGEM®-T Easy Vector Systems

pGEM®-T Easy Vector Systems

Convenience and Flexibility for Cloning PCR Products

  • Three options for removing the insert with a single digest (BstZI, EcoRI or NotI )
  • Ligation can be completed in 1 hour at room temperature
  • Available with or without competent cells

Please Enquire

Description

Size

PCR Cloning with Blue/White Selection and Easy Insert Excision

The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation.

T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication.

The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.

There is no GenBank® Accession Number for the pGEM®-T Easy Vector. The parent vector is linearized at the position indicated by * in this pGEM®-T Easy Vector Sequence and a “T” is added at each end.

Protocols

Complete Protocol

pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual

Quick Protocols

pGEM T and pGEM T Easy Vector Systems FB033

Specifications

What’s in the box?

Item/ Catalog number selected: A1360 Part # Size Concentration Available Separately
pGEM®-T Easy Vector A137A 1 × 1.2μg 50ng/μl
Control Insert DNA A363A 1 × 12μl
2X Rapid Ligation Buffer C671A 1 × 200μl View Product
T4 DNA Ligase M180A 1 × 100u

SDS For A1360

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

What’s in the box?

Item/ Catalog number selected: A1360 Part # Size Concentration Available Separately
pGEM®-T Easy Vector A137A 1 × 1.2μg 50ng/μl
Control Insert DNA A363A 1 × 12μl
T4 DNA Ligase M180A 1 × 100u
2X Rapid Ligation Buffer C671A 1 × 200μl View Product
JM109 Competent Cells, High Efficiency L200A 6 × 200μl

SDS For  A1380

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.