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NotI

  • Capable of digesting DNA in 15 minutes or less
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications
  • High concentration product no longer available

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Description

10μ/μl

Catalog number selected: R6431

200u

Catalog number selected: R6431

1,000u

Catalog number selected: R6435

Cut Site:

GCGGCC  GC
CG  CCGGCG

Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated.

Incubation Conditions: Buffer D. 37°C.

Source: Nocardia otitidis-caviarum.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 0.1% Triton® X-100, 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™
<10% 10–25% 25–50% 100% 25–50%
Frequency of Cutting:
λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
0 7 0 0 0 0

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

You are viewing: R6435 Change Configuration
Item Part # Size Concentration Available Separately
Buffer D 10X Buffer R004A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
NotI R643E 1 × 1,000u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6431 Change Configuration
Item Part # Size Concentration Available Separately
Buffer D 10X Buffer R004A 1 × 1ml
Bovine Serum Albumin, Acetylated R396D 1 × 150μl 10mg/ml View Product
NotI R643A 1 × 200u
MULTI-CORE™ 10X Buffer R999A 1 × 0.25ml View Product

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions