Description
Automated Purification of Total Nucleic Acid from Viral Sample Types
Emergence of new viral diseases, including the coronavirus SARS-CoV-2 (COVID 19), MERS (2015) and SARS (2003) outbreaks, highlight the need for fast methods to detect and identify target viruses at scale. The Maxwell® HT Viral TNA Kit can be used for high-throughput, automated extraction of RNA and DNA from several sample types, including universal transport medium, serum, plasma and others. Samples are processed in 96-well plates using magnetic separation. The Maxwell® HT purification kit can also be used in manual protocols using a multichannel pipettor.
Protocols are available for automated platforms and manual use. These are provided in the Application Notes linked below.
Inactivated HIV-1 (5,000IU/ml) and 500IU/ml of inactivated Hepatitis B virus (HBV) were isolated from a 300μl sample of human plasma using the Maxwell® HT Viral TNA chemistry automated on a Tecan Freedom EVO® and compared with performance of the Qiagen QIAamp® MinElute® kit. Quantitation was conducted using PathID™ RT-qPCR (HIV-1) and Plexor® RT-qPCR (HBV).
Maxwell® HT RNA and DNA Extraction Protocol Overview
Viral Nucleic Acid Purification: Automated Protocols
Viral Nucleic Acid Purification: Manual Protocols
What’s in the box?
| Item - Catalog number: AX2340 | Part # | Size | Available Separately |
| MagneSil® RED | A164B | 1 × 60ml | View Product |
| 4/40 Wash Solution | A2221 | 1 × 115ml | View Product |
| Proteinase K (PK) Solution | MC500E | 1 × 16ml | View Product |
| Alcohol Wash, Blood | MD1411 | 1 × 70ml | View Product |
| Nuclease-Free Water | P1195 | 1 × 150ml | View Product |
| Lysis Buffer | MC5018 | 1 × 160ml |
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
Patents and Disclaimers
U.S. Pat. No. 7,329,488 and S. Korean Pat. No. 100483684.
