Description
Automated RNA Extraction from 1–16 Plant Tissue Samples in 60 Minutes
Faster RNA Purification, Less Hands-On Time and More Efficient Workflows
The Maxwell® 16 LEV Plant RNA Kit is used with the Maxwell® 16 Research Instrument (Cat.# AS2000) to provide an easy method for efficient, automated purification of RNA from plant tissue samples. The Maxwell® 16 Instrument is supplied with preprogrammed purification methods and is designed for use with the predispensed reagent cartridges supplied in the Maxwell® 16 LEV Plant RNA Kit. The instrument can process from 1 to 16 samples in under an hour, and the purified RNA can be used directly in downstream applications including real-time RT-qPCR, gel electrophoresis, microarrays and sequencing (e.g., NGS and Sanger).
- Consistent processing and higher yields mean less re-isolation of samples, reducing costs and increasing the reliability of your results.
- No organic extraction and minimal preprocessing required.
RNA was extracted from 40mg of Arabidopsis, corn and soybean leaf tissues (n=6 for each sample type) using the Maxwell® RSC Plant RNA Kit and eluted in 50µl. Eluted RNA was quantified using the QuantiFluor® RNA Dye and the Quantus™ Fluorometer.
RNA was extracted from 40mg of fresh leaf tissues (n=4 for each sample type) using the Maxwell® 16 Plant RNA kit and eluted in 50µl. Yield was determined by spectroscopy. A260/A280 ratios above 1.8, and A260/A230 ratios between 1.8 and 2.4 were achieved for all samples.
RNA was extracted from 40mg of plant leaf tissue from the indicated species, eluted in 50µl and quantitated by RT-qPCR using species-specific primer/probe sets. The 1:10 dilution for all three species indicates that there is no inhibition based on volume input.
Inhibition of amplification of extracted RNA: Comparison to an Internal Positive Control. RNA samples (n=4), purified from leaves of the indicated plants, were added to a TaqMan® Exogenous Internal Positive Control (IPC) reaction. A ΔCt value of zero indicates no inhibition, and a ΔCt of two or below is considered acceptable. The observed ΔCt values for the Maxwell® 16 LEV Plant RNA samples indicate minimal inhibition.Protocols
What’s in the box?
| Item - Catalog number: AS1430 | Part # | Size | Available Separately |
| 1-Thioglycerol | A208B | 1 × 900μl | |
| Maxwell® RSC Cartridge (RSCO) | AS174A | 48 × 1 each | |
| LEV Plungers | AS610A | 1 × 50/pk | View Product |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product |
| Blue Dye | C888A | 1 × 50μl | |
| Lysis Buffer | MC501C | 1 × 20ml | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
| Homogenization Solution | Z305H | 1 × 30ml | |
| DNase I (lyophilized) | Z358A | 2 × 1 vial |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Patents and Disclaimers
U.S. Pat. No. 6,855,499 and other patents.
