Description
Efficient, Automated Purification of DNA from Plant Tissues
Hands-Free Purification of PCR-Ready DNA from 1–16 Plant Samples
The Maxwell® 16 LEV Plant DNA Kit is used with the Maxwell® 16 Instrument to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from plant tissue samples, including corn, soybean and Arabidopsis. DNA can be purified from up to 16 samples in less than 60 minutes with minimal preprocessing and no organic extractions. The purified DNA contains minimal contaminants and enzyme inhibitors, and is ready for use in downstream applications such as real-time PCR, gel electrophoresis, microarrays and sequencing (e.g., NGS and Sanger).
The Maxwell® Plant DNA Kits rapidly produce high-quality DNA for use in sequencing and qPCR-based applications in plant genetics and molecular plant breeding, including gene expression profiling, analysis of genetic variation in plants, and genotype/phenotype analysis.
High DNA Yield from a Range of Plant Species
The cellulose-based particles in the Maxwell® Kits have higher binding capacity than silica-based DNA purification methods, giving consistently high yields. The low elution volume allows generation of more concentrated DNA than other purification methods.
The example data shown here compares the DNA yield from soybean, wheat, corn and Arabidopsis using the Maxwell® 16 LEV Plant DNA Kit and a competitor method. DNA was quantified using the QuantiFluor® dsDNA Dye and the Quantus™ Fluorometer.
Low Elution Volume Allows Purification of More Concentrated DNA
These examples show how the low elution volume used in the Maxwell® 16 LEV Plant Kit method results in highly concentrated purified DNA.
DNA was extracted from flash-frozen leaf samples (~20mg each) using either a CTAB protocol, the Maxwell® Plant DNA Kit or the Qiagen DNeasy® Plant Mini Kit. Samples were bead-beaten for 40 seconds at 6m/s and processed according to each manufacturer’s protocol. Maxwell® samples were eluted in 50µl, Qiagen samples in 200µl, and CTAB samples in 100µl. Species-specific primers were used for quantitation by qPCR and a standard curve was generated for each plant species using control DNA (Zyagen Life Science Products) with the GoTaq® qPCR System (Cat. #AS6001). For qPCR, each sample was run in a 50µl reaction with 2µl input DNA. Standard deviations represent N=9 for Arabidopsis and N=6 for corn
Minimal Downstream PCR Inhibition
DNA purified with the Maxwell® Plant DNA Kits is ready for use in downstream applications, including qPCR. PCR using DNA purified with the Maxwell® Plant DNA Kits shows no significant evidence of inhibitors.
In this experiment, samples (n=5) purified from various plant species using the Maxwell® 16 LEV Plant DNA Kit and the indicated preprocessing methods were added to a TaqMan® Exogenous Internal Positive Control (IPC) reaction. IPC reagents contain a pre-optimized internal positive control with pre-designed primers and TaqMan® probe. Samples can be added to IPC reactions to determine whether PCR inhibitors are present. A ΔCt value of zero indicates no inhibition, and a ΔCt of 2 or below is considered acceptable. The observed ΔCt values for the Maxwell® 16 LEV Plant DNA samples indicate minimal inhibition.
These examples show how the low elution volume used in the Maxwell® 16 LEV Plant Kit method results in highly concentrated purified DNA.
Protocols
Complete Protocol
Quick Protocol
What’s in the box?
| Item/ Catalog number selected: AS1420 | Part # | Size | Concentration | Available Separately |
| Tail Lysis Buffer (TLA) | A509C | 1 × 25ml | View Product | |
| RNase A Solution | A797C | 1 × 1ml | 4mg/ml | View Product |
| Elution Buffer | A828D | 1 × 20ml | View Product | |
| Maxwell® 16 LEV Cartridge (MCD) | AS127A | 48 × 1 each | ||
| LEV Plungers | AS610A | 1 × 50/pk | View Product | |
| Elution Tubes (0.5ml) | AS620A | 1 × 50/pk | View Product | |
| Nuclease-Free Water | P119C | 1 × 25ml | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
