Description
Size: 10,000u
Catalog number selected: M1701
Size: 50,000u
Catalog number selected: M1705
RNA-Dependent DNA Polymerase for cDNA Synthesis
RNA-Dependent DNA Polymerase for cDNA Synthesis
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Advantages
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
- May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
Protocols
What’s in the box?
| Item/ size 10,000u : Catalog number selected: M1701 | Part # | Size |
| M-MLV Reverse Transcriptase | M170A | 1 × 10,000u |
| M-MLV RT 5X Buffer | M531A | 1 × 1ml |
| Item/ size 50,000u: Catalog number selected: M1705 | Part # | Size |
| M-MLV Reverse Transcriptase | M170B | 1 × 50,000u |
| M-MLV RT 5X Buffer | M531A | 2 × 1ml |
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Storage Conditions
What’s in the box?
| Item/ size: 2 × 1ml Catalog number selected: M5313 | Part # | Size |
| M-MLV RT 5X Buffer | M531A | 2 × 1ml |
SDS For M5313
Download SDSUse Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated
Storage Conditions
