Description
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.
For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.
JM109 Competent Cells are prepared according to a modified procedure of Hanahan.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
Reference
- Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.
Protocols
Complete Protocol
What’s in the box?
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| Item | Part # | Size |
| JM109 Competent Cells, Subcloning Efficiency | L100A | 5 Ă— 200ÎĽl |
| Competent Cells Control DNA | L107A | 1 Ă— 30ÎĽl |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Â
| Item | Part # | Size |
| Single-Use JM109 Competent Cells, >108cfu/µg | L200B | 20 × 50μl |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
Â
| Item | Part # | Size |
| Competent Cells Control DNA | L107A | 1 Ă— 30ÎĽl |
| JM109 Competent Cells, High Efficiency | L200A | 5 Ă— 200ÎĽl |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
