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JM109 Competent Cells

JM109 Competent Cells

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Description

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Size

1ml (5 x 200µl)

Catalog number selected: L2001

Size

1ml (20 x 50µl)

Catalog number selected: L2005

 

Size

1ml (5 x 200µl)

Catalog number selected:   L1001

JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

JM109 Competent Cells are prepared according to a modified procedure of Hanahan.

JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].

Reference

  1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.

Protocols

Complete Protocol

E. coli Competent Cells Technical Bulletin

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Quick Protocols

E Coli Competent Cells Quick Protocol FB035

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Specifications

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You are viewing: L1001 Change Configuration

 

Item Part # Size
JM109 Competent Cells, Subcloning Efficiency L100A 5 × 200μl
Competent Cells Control DNA L107A 1 × 30μl

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: L2005 Change Configuration

 

Item Part # Size
Single-Use JM109 Competent Cells, >108cfu/µg L200B 20 × 50μl

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: L2001 Change Configuration

 

Item Part # Size
Competent Cells Control DNA L107A 1 × 30μl
JM109 Competent Cells, High Efficiency L200A 5 × 200μl

SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions