Description
Cut Site:
CTCTCÂ Â Â TTAAâ–ĽGGTAGC
GAGAGâ–˛AATTÂ Â CCATCG
Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.
Incubation Conditions: I-PpoI Buffer. 37°C.
Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.
Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | ||||
| A | B | C | D | I-PPoI |
| 10–25% | 25–50% | 25–50% | 25–50% | 100% |
Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.
Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.
Protocols
Complete Protocol
Â
 Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Bovine Serum Albumin, Acetylated | R396A | 1 Ă— 1,000ÎĽl | 10mg/ml | View Product |
| I-PpoI | R703A | 1 Ă— 10,000u | ||
| I-PpoI Positive Control Plasmid | R707A | 1 Ă— 10ÎĽg | ||
| I-PpoI 10X Buffer | R994A | 1 Ă— 1ml |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
