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I-PpoI (Intron-Encoded Endonuclease)

I-PpoI (Intron-Encoded Endonuclease)

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Description

8-12μ/μl

Catalog number selected:  R7103

250u

Catalog number selected:  R7103

Cut Site:

CTCTC    TTAAGGTAGC
GAGAGAATT   CCATCG

Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.

Incubation Conditions: I-PpoI Buffer. 37°C.

Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:
A B C D I-PPoI
10–25% 25–50% 25–50% 25–50% 100%

Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

Specifications

What’s in the box?

Item Part # Size Concentration Available Separately
Bovine Serum Albumin, Acetylated R396A 1 × 1,000μl 10mg/ml View Product
I-PpoI R703A 1 × 10,000u
I-PpoI Positive Control Plasmid R707A 1 × 10μg
I-PpoI 10X Buffer R994A 1 × 1ml

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions