Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.
Incubation Conditions: I-PpoI Buffer. 37°C.
Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.
Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
|Percent Activity in 4-CORE® Buffer:|
Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.
Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.
What’s in the box?
|Item||Part #||Size||Concentration||Available Separately|
|Bovine Serum Albumin, Acetylated||R396A||1 × 1,000μl||10mg/ml||View Product|
|I-PpoI||R703A||1 × 10,000u|
|I-PpoI Positive Control Plasmid||R707A||1 × 10μg|
|I-PpoI 10X Buffer||R994A||1 × 1ml|
For Research Use Only. Not for Use in Diagnostic Procedures.