Description
Cut Site:
G▼GATC C
C CTAG▲G
Usage Notes:
- Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
- Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
Incubation Conditions: Buffer E. 37°C.
Source: Bacillus amyloliquefaciens H.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | E | MULTI-CORE™ |
| 75–100%* | 75–100% | 75–100% | 50–75% | 100% | 75–100% |
| *Buffer A is not recommended due to potential star activity. | |||||
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 5 | 3 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
You are viewing: R6021 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer E 10X Buffer | R005A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| BamHI | R602A | 1 × 2,500u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
What’s in the box?
You are viewing: R6025 & R6045 Change Configuration
| Item | Part # | Size | Concentration | Available Separately |
| Buffer E 10X Buffer | R005A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396E | 1 × 750μl | 10mg/ml | View Product |
| BamHI (HC) | R402A | 1 × 12,500u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
