Description
Cut Site:
A▼CCGG T
T GGCC▲A
Usage Note: Ends generated with AgeI can be directly ligated to ends generated using AccIII, NgoMIV or XmaI. The AgeI recognition site is not regenerated in the ligation product.
Incubation Conditions: Buffer K. 37°C.
Source: Agrobacterium gelatinovorum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol, 0.5mg/ml BSA.
| Percent Activity in 4-CORE® Buffer: | |||||
| A | B | C | D | K | MULTI-CORE™ |
| 25–50% | 25–50% | 25–50% | 50–75% | 100% | 100% |
| Frequency of Cutting: | |||||
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
| 13 | 5 | 0 | 0 | 0 | 0 |
Protocols
Complete Protocol
Restriction Enzyme Protocol
What’s in the box?
| Item | Part # | Size | Concentration | Available Separately |
| Buffer K 10X Buffer | R010A | 1 × 1ml | ||
| Bovine Serum Albumin, Acetylated | R396D | 1 × 150μl | 10mg/ml | View Product |
| AgeI | R725A | 1 × 100u | ||
| MULTI-CORE™ 10X Buffer | R999A | 1 × 0.25ml | View Product |
SDS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
