Why Quantitate on Quantus Fluorometer ?
- Save Your VALUABLE TIME and PCR REAGENTS By Knowing What You Have Rather Than Repeating Experiments.Each time there is a new purified DNA Sample, the chances that you have the sample quantity as are lessened. Consequently without knowing the DNA Concentration of the sample you are using, the amount of input DNA cannot be guaranteed and experiments may have to be repeated.
- Quantitation by Fluorescence provides certainty that you are using the Right DNA (Single Stranded DNA or Double Stranded DNA) for PCR
- UV Absorbance measurements cannot distinguish between ssDNA and dsDNA. Using a species-specific fluorescent dye like the QuantiFluor® ssDNA System and the QuantiFluor® dsDNA System would ensure you are only quantitating the
desired DNA molecules of interest.
MORE ABOUT QUANTUS FLUOROMETER
- TWO FLUORESCENT CHANNELS:
Red: Ex: 640nm shortpass (wavelengths up to 640nm); Em: 660–720nm
Blue: Ex: 495nm shortpass (wavelengths up to 495nm); Em: 510–580nm
- EASILY CUSTOMIZE SAMPLE NAMES, VIEW
CONCENTRATION RESULTS, RAW VALUES,
SAMPLE VOLUMES AND A QUALITY CHECK.
- INTUITIVE NAVIGATION
- NGS: QUANTUS TM Fluorometer is recommended for NGS
and compatible with Illumina (HiSeq/miSeq), Roche (454)
and LifeTech (ion Torrent and ion Proton) Systems.
- Calculates concentration automatically.
Store the last 50 readings and transfer data in real time.
Fluorescence-based nucleic acid quantification methods are more sensitive than absorbance-based methods, allowing you to quantitate low-level DNA samples accurately.
Custom protocols can be created for the Quantus™ Fluorometer to detect even lower amounts.
Broad Dynamic Range
Complement your absorbance-based methods and quantify your target molecule over a wider range of concentrations.
Quantitation of more dilute samples is possible using more input dsDNA per assay.
Calibration and Sample Measurement
The calibration process eliminates the need to generate a standard curve for each set of unknown samples. Simply measure the fluorescence of your blank sample and the standard that represents the highest point on your standard curve. The Quantus™ Fluorometer saves the calibration data for future measurements.
No computer is necessary to use the Quantus™ Fluorometer, but after installing the free Quantus™ Software you can automatically transfer your quantitation results from the Quantus™ Fluorometer to a personal computer in real time. Export results easily into Microsoft® Excel.
Quantus™ Fluorometer Calibration
Quantus™ Fluorometer Sample Measurement
Quantus™ Fluorometer Results & History
The high sensitivity makes the Quantus™ Fluorometer perfect when working with samples types such as formalin-fixed paraffin-embedded (FFPE) tissue that contain low levels of nucleic acid.
You can confidently quantify nucleic acid from FFPE tissues and proceed to your downstream assays, knowing that there is DNA or RNA present.
Successful and accurate quantification leads to higher success in downstream assays such as PCR, cloning, transfection and next-generation sequencing.
|Size||22.7 × 11.5 × 4.5cm (L × W × H)|
|Linear Dynamic Range||Five orders of magnitude, assay-dependent|
|Detectors||Solid state silicon sensor|
|Excitation Filters||Red: 640nm shortpass (wavelengths up to 640nm)
Blue: 495nm shortpass (wavelengths up to 495nm)
|Emission Filters||Red: 660–720nm
|Calibration Type||Single-point (blank and standard)|
|Sample Tube||We recommend Axygen thin-walled PCR tubes (Axygen Cat.# PCR-05-C, available from Fisher or VWR)|
|Automatic Sleep Mode||After 1 hour of inactivity|
|Power Requirements||5V, 0.2A Maximum|
|Operating System Requirements
(for optional Quantus™ Software)
|Windows® 10, Windows® 8, Windows® 7 (32 or 64 bit)
.NET Framework 4.0 or later
At least one USB port available
Frequently Asked Questions
What is the Quantus™ Fluorometer?
The Quantus™ Fluorometer is an easy-to-use and affordable single-tube fluorometer. The compact instrument offers intuitive navigation and a large 3-inch liquid crystal display screen. The fluorometer is preprogrammed with protocols for single-stranded DNA, double-stranded DNA and RNA quantitation using the QuantiFluor® Dyes but is also compatible with other fluorescent assays.
What are the excitation and emission wavelengths?
The Quantus™ Fluorometer is equipped with two fluorescence channels for nucleic acid and protein quantitation:
Blue fluorescence channel: Excitation 495nm shortpass (wavelengths up to 495nm), emission 510–580nm.
Red fluorescence channel: Excitation 640nm shortpass (wavelengths up to 640nm), emission 660–720nm.
How sensitive is the Quantus™ Fluorometer?
When used with the QuantiFluor® Dyes, the Quantus™ Fluorometer provides sensitivities that are up to 40,000 times higher than that of absorbance-based quantitation, depending on the assay used. As little as 50pg/ml dsDNA can be detected using the standard QuantiFluor® dsDNA Dye protocol. Custom protocols can be created to detect even lower amounts (as little as 1pg/ml dsDNA). The instrument has a linear range that encompasses five orders of magnitude.
Is a computer required to use the Quantus™ Fluorometer?
No. The Quantus™ Fluorometer is designed to operate as a standalone instrument. The Quantus™ Fluorometer displays fluorescence measurements in real time but also saves and displays the 50 most recent measurements in the results history. However, the fluorometer can be connected to a computer to transfer data in real time after installing the free Quantus™ Software.
What are the operating system requirements for the optional Quantus™ Software?
To download and use the Quantus™ Software, your computer must have the Windows®10, Windows® 8 or Windows® 7 (32 or 64 bit) operating system, a .NET Framework 4.0 or later and at least one USB port available.
What tubes are compatible with the Quantus™ Fluorometer?
The Quantus™ Fluorometer accepts 0.5ml PCR tubes. We recommend thin-walled PCR tubes (Axygen thin-walled PCR tubes, Axygen Cat.# PCR-05-C, available from Fisher or VWR) for the best optical clarity.
What are the power requirements for the Quantus™ Fluorometer?
The fluorometer requires a power source of 5V, 0.2A maximum. The Quantus™ Fluorometer is supplied with a USB Cord to plug the instrument into a computer and a USB Power Supply and Outlet Adapter Kit, which includes four adapters, to plug the instrument into the wall outlet.
How do I calibrate the Quantus™ Fluorometer, and how often should I recalibrate the instrument?
To calibrate, simply measure the fluorescence of your blank sample and the standard that represents the highest point on your standard curve. The Quantus™ Fluorometer saves the calibration data for future measurements. The fluorometer must be calibrated prior to the first use of a protocol. If a protocol that has never been calibrated is selected, the Calibration screen will appear automatically. Once the protocol of interest is calibrated, the Quantus™ Fluorometer will save the calibration values for future use and will not require recalibration. However, we recommend performing a calibration with each new lot of dye reagent to ensure the most accurate measurement.
Does purchase of the Quantus™ Fluorometer include a warranty?
Yes. The Quantus™ Fluorometer comes with a 1-year warranty upon purchase of the instrument. Warranty coverage includes all parts, labor and shipping to and from the repair location.
QuantiFluor is a registered trademark of Promega Corporation. Quantus is a trademark of Promega Corporation. Windows is a registered trademark of Microsoft Corporation.