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Universal RiboClone™ cDNA Synthesis System

  • Reagents to synthesize double-stranded cDNA from up to 40µg of mRNA
  • Oligo(dT)15 Primer and Random Primers included for choice of priming methods
  • Materials provided for 10 adaptor ligation reactions

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Description

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Catalog number selected: C4360

  • Reagents to synthesize double-stranded cDNA from up to 40µg of mRNA
  • Oligo(dT)15 Primer and Random Primers included for choice of priming methods
  • Materials provided for 10 adaptor ligation reactions

 

The Universal RiboClone™ cDNA Synthesis System is used for double-stranded cDNA synthesis from RNA. The system also includes materials for ligation of the cDNA into a suitable vector. First-strand synthesis is driven by Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and either Random Primers or Oligo(dT)15 Primer. This is followed directly by second-strand synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to create blunt ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and addition of EcoRI Adaptors. The resulting cDNA preparation is ready for cloning into a suitable vector.

Protocols

Complete Protocol

Universal RiboClone™ cDNA Synthesis System Technical Manual

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What’s in the box?

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SDS For C4360

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.