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Terminal Deoxynucleotidyl Transferase, Recombinant

Adds Mononucleotides to the Terminal 3´-OH of ssDNA and Releases PPi

  • Adds mononucleotide tails to any type of 3´ end (3´ and 5´ overhangs or blunt ends) due to the presence of 1mM CoCl2 in the reaction buffer
  • Quality tested for labeling of apoptotic DNA ends using the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235 for each lot of enzyme
  • Supplied at a concentration of 30u/μl

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Description

Size

Size: 300u
Catalog number: M1871

Size: 1,500u
Catalog number: M1875

Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3´ ends with varying degrees of efficiency. TdT is provided with 5X Reaction Buffer: 500mM cacodylate buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT.

 

Applications

  • Tailing reactions to add complementary homopolymer tails to DNA vectors and cDNA.
  • 3´ end-labeling.
  • TUNEL assays.

Storage Buffer: 50mM potassium phosphate (pH 6.4), 100mM NaCl, 1mM β-mercaptoethanol, 0.1% Tween® 20 and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, endonuclease, DNase, RNase, 3´ end-labeling, apoptotic DNA end-labeling.

Unit Definition: One unit of activity catalyzes the transfer of 0.5 picomoles of ddATP to oligo(dT)16 per minute at 37°C in 1X Terminal Transferase Buffer. The resulting oligo(dT)17 is measured by HPLC.

Protocols

Complete Protocol

Terminal Deoxynucleotidyl Transferase, Recombinant Protocol


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What’s in the box?
Size: 300u

[wpdatatable id=389 table_view=regular]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

What’s in the box?
Size: 1,500u

[wpdatatable id=390 table_view=regular]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB