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TeloPrime Full-Length cDNA Amplification Kit V2

  • Full-length cDNA generation with high yield
  • Exceptional 5‘ cap specificity
  • Ideal for long-read NGS library generation
    (e.g. PacBio™ and Oxford Nanopore™)
  • 1 ng – 2 μg total RNA input
  • Flexible protocol enables use of custom primers
    for reverse transcription

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Description

The TeloPrime Full-Length cDNA Amplification Kit V2 is an all-in-one protocol for generating full-length cDNA from capped and polyadenylated RNAs. cDNA generated with TeloPrime is suitable for various downstream applications such as e.g., NGS library generation (incl. PacBio), probes, RACE, and cloning.

Features and Application:

  • NGS library generation (including PacBio)
  • RACE
  • generation of probes
  • cloning

Kit Content:

  • Reagent Box
  • Buffers and Purification Columns

013 (TeloPrime Full-Length cDNA Amplification Kit),

018 (TeloPrime PCR Add-on Kit).

TeloPrime Full-Length cDNA Amplification Kit V2

SARS-CoV-2_Icon   This product can be used for SARS-CoV-2 research.

The TeloPrime Full-Length cDNA Amplification Kit V2 is an all-in-one protocol for generating full-length cDNA from total RNA.

Full-Length cDNA Synthesis

Based on Lexogen´s unique Cap-Dependent Linker Ligation (CDLL) and long reverse transcription (long RT) technology, it is highly selective for full-length RNA molecules that are both capped and polyadenylated.

For Multiple Downstream Applications

The full-length cDNA products can be used for various downstream applications such as NGS, RACE, cloning, microarray probes, and normalization. It enables the detection and correct quantification of splice variants and their true transcription start- and end-sites, in both short and long mRNA molecules. For further full-length or gene-specific PCRs, Lexogen offers a TeloPrime PCR Add-on Kit V2 (Cat.No.018.16) containing 16 rxn. This kit contains the PCR Forward and Reverse Primer separately,hence they can be alternatively substituted with the gene-specific primer of interest.

Exceptional Elimination of cDNA from Degraded RNA

Based on the CDLL technology the ligation of the 5’ linker to the 3’ end of the cDNA takes place in a highly cap-dependent manner, providing an exceptional cap-specificity and eliminating cDNA products from degraded mRNA.

teloprimediagram

In an mRNA-Seq experiment, mRNAs were tagged at their 5’ end using TeloPrime, Template-Switch (TS) or Oligo Capping (OC). Transcript start sites (TSS) were mapped to the mouse genome. The accumulated TSS read coverage is plotted versus the normalized annotated transcript length to show relative TSS mapping for the top 500 expressed genes.

Workflow

Downloads

TeloPrime Full-Length cDNA Amplification Kit V2

pdf  Product Flyer – Release of TeloPrime V2 (20.12.2018)

pdf  013UG022V0200 – User Guide – update 22.11.2018

   013MS034V0201 – MSDS Information for TeloPrime Full-Length cDNA Amplification Kit V2 – update 18.12.2018

Product Items

Catalog Nr. Product Name
013.08 TeloPrime Full-Length cDNA Amplification Kit, 8 preps
013.24 TeloPrime Full-Length cDNA Amplification Kit, 24 preps