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T4 Polynucleotide Kinase

Transfers the γ-Phosphate from ATP to the 5´ End of Nucleotides

  • Can be inactivated by heating at 68°C for 10 minutes
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Labels the 5´ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
  • Supplied at a concentration of 5–10u/μl

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Description

Catalog Number

Size: 100u
Catalog number: M4101

Size: 1,000u
Catalog number: M4103

T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation and cloning. T4 PNK is provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT.

Storage Buffer: 20mM Tris-HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP and 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, DNase, RNase, endonuclease, end-labeling, blue/white cloning assay.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [γ-32P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 5mM DTT, 0.1mM [γ-32P] ATP and 0.5mM 5´-OH polynucleotide end concentration.

Protocols

Complete Protocol

T4 Polynucleotide Kinase Technical Bulletin

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What’s in the box?
Size: 100u

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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What’s in the box?
Size: 1,000u

[wpdatatable id=388 table_view=regular]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB