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T4 DNA Polymerase

Synthesizes DNA 5´→3´ from a Primed ssDNA Template

  • High fidelity enzyme of choice for applications where misincorporation is a concern
  • Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
  • Heat inactivated by heating at 75°C for 10 minutes
  • Supplied at a concentration of 5–10u/μl

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Description

Size: 100u
Catalog number: M4211

Size: 500u
Catalog number: M4215

T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill in 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs or in vitro mutagenesis. The enzyme is provided with 10X Reaction Buffer: 250mM Tris-acetate (pH 7.7), 1M potassium acetate, 100mM magnesium acetate and 10mM DTT.

 

References

  1. Challberg, M.D. and Englund, P.T. (1980) Meth. Enzymol. 65, 39–43.
  2. Burd, J.F. and Wells, R.D. (1974) J. Biol. Chem. 249, 7094–801.
  3. Kunkel, T.A., Loeb, L.A. and Goodman, M.F. (1984) J. Biol. Chem. 259, 1539–45.

Storage Buffer: 200mM potassium phosphate (pH 6.5 at 25°C), 2mM DTT and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, endonuclease.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 55nmol of dTTP into acid-precipitable material in 30 minutes at 39°C using poly(dA):oligo(dT) as a substrate.

Protocols

Complete Protocol

T4 DNA Polymerase Protocol
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What’s in the box?
Size: 100u

Item Part # Size
T4 DNA Polymerase M421A 1 × 100u
T4 DNA Polymerase 10X Reaction Buffer M831A 1 × 1ml

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

What’s in the box?
Size: 500u

Item Part # Size
T4 DNA Polymerase M421F 1 ×
500u
T4 DNA Polymerase 10X Reaction Buffer M831A 1 × 1ml

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB