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T4 DNA Ligase

Joins Two DNA Strands with Cohesive or Blunt Ends

  • Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
  • Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
  • Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule

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Description

Size

concentration: 1–3u/µl
Size: 100u (Weiss units)
Catalog number: M1801

concentration: 1–3u/µl
Size: 500u (Weiss units)
Catalog number: M1804

concentration: 10–20u/µl
Size: 500u (Weiss units)
Catalog number: M1794

T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

 

Reference

  1. Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.

Note: Units listed are Weiss units.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

Protocols

Complete Protocol

T4 DNA Ligase, Blue/White Cloning Qualified Protocol


[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

What’s in the box?
concentration: 1–3u/µl
Size: 100u (Weiss units)

[wpdatatable id=393 table_view=regular]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

 

What’s in the box?
concentration: 1–3u/µl
Size: 500u (Weiss units)

[wpdatatable id=394 table_view=regular]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

 

What’s in the box?
concentration: 10–20u/µl
Size: 500u (Weiss units)

[wpdatatable id=395 table_view=regular]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB