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Single-Stranded DNA Binding Protein

Cooperatively Binds ssDNA with High Affinity

  • Consists of four identical 18.9kDa subunits
  • Does not bind well to double-stranded DNA
  • Supplied at a concentration of 1–5µg/μl

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Description

Size: 100μg
Catalog number: M3011

E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits that cooperatively bind to single-stranded DNA with high affinity. This protein is involved in DNA replication and in recombination in vivo.

Applications

  • Studying DNA replication and recombination.
  • DNA sequencing using the primer walking strategy with contiguous oligonucleotides.

References

  1. Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4274–8.
  2. Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55, 103–36.
  3. Krauss, G. et al. (1981) Biochemistry 20, 5346–52.
  4. Weiner, J.H. et al. (1975) J. Biol. Chem. 250, 1972–80.

Storage Buffer: 50mM Tris-HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% (v/v) glycerol.

Source: E. coli.

QC Tests: Protein concentration, DNase, RNase, SDS-PAGE/purity, endonuclease.

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What’s in the box?
Size: 100μg

Item Part # Size
Single-Stranded DNA Binding Protein M301A 1 × 100μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB