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SgfI

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Description

8-12μ/μl

Catalog number selected:  R7103

250u

Catalog number selected:  R7103

Cut Site:

GCG  ATCGC
CGCTA  GCG

Notes:

  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. Sensitive to CpG methylation.
  3. SgfI is inactivated by freezing at –70°C.
  4. Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Incubation Conditions: Buffer C. 37°C.

Source: Streptomyces fulvissimis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions