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Ribonuclease H

Endonuclease that Specifically Hydrolyzes the Phosphodiester Bonds of RNA Hybridized to DNA

  • Removes the RNA strand prior to second-strand cDNA synthesis
  • Analyzes in vitro polyadenylation reaction products
  • Supplied at a concentration of 0.5–2u/μl

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Description

Catalog number

Size: 50u
Catalog number: M4281

Size: 250u
Catalog number: M4285

Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3´-OH and 5´-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. This endonuclease can be used in applications such as microarrays.

Reference

  1. Goodwin, E.C. and Rottman, F.M. (1992) Nucl. Acids Res. 20, 916.

Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 50% (v/v) glycerol, 0.2mg/ml BSA.

Source: Recombinant E. coli strain.

QC Tests: Activity, DNase, RNase, endonuclease, cDNA synthesis.

Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).

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What’s in the box?
Size: 50u

Item Part # Size
RNase H M428A 1 × 50u

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

 

What’s in the box?
Size: 250u

Item Part # Size
RNase H M428C 1 × 250u

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB