Molecular Products Co.

Search
customer-support

Call Us Now:

0334-7752577

RiboMAX™ Large Scale RNA Production Systems

Please Enquire

Description

Size

Catalog number : P1280

Catalog number :  P1300

More RNA from Transcription Reactions

The RiboMAX™ Large Scale RNA Production System synthesizes large quantities of capped or uncapped RNA transcripts suitable for in vitro or in vivo translation. RNA synthesized with the RiboMAX™ Large Scale RNA Production System performs better for in vitro rabbit reticulocyte translation systems than RNA synthesized by standard methods. This is especially notable with high RNA concentrations that normally inhibit in vitro translation. With the reduction of components inhibitory to translation, there may be an advantage for other applications requiring biologically active RNA.

The RiboMAX™ Large Scale RNA Production System consistently produces about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System. Because the RiboMAX™ Systems produce such large quantities of RNA, they are not recommended for generation of high-specific-activity RNA probes, due to the cost requirement of radiolabeled nucleotides.

RiboMAX™ System reactions differ from those of the Riboprobe® Systems in three main ways: 1) a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer; 2) rNTP and magnesium concentrations are elevated at levels appropriate for either SP6 or T7 RNA polymerase; and 3) inorganic pyrophosphatase is included in the reaction.

Note: Use of the RiboMAX™ System for production of capped transcripts requires separate purchase of the Ribo m7G Cap Analog (Cat.# P1711).

RiboMAX™ System Applications:

  • Generation of dsRNA and long RNAs for nonmammalian RNA interference studies.
  • In vitro synthesis of tRNA and rRNA, and RNA virus genomes and ribozymes.
  • Production of substrates for RNA splicing, studies of RNA secondary structure and RNA:protein interactions.
  • Synthesis of antisense RNA

References

  1. Gurevich, V.V. et al. (1991) Anal. Biochem. 195, 207–13.
  2. Beckler, G.S. (1992) Promega Notes 39, 12–6.
RiboMax produces more RNA than standard in vitro transcription protocols.
Effect of pGEM®-luc RNA concentration on in vitro translation. The indicated amounts of pGEM®-luc RNA transcripts were used to program 50µl of Rabbit Reticulocyte Lysate in vitro translation reactions. Expression of functional luciferase was measured as follows: 2.5µl of each reaction was mixed with an equal volume of Luciferase Assay Reagent and luminescence measured for 10 seconds in a luminometer.

Protocols

Complete Protocol

RiboMAX™ Large Scale RNA Production Systems—SP6 and T7 Technical Bulletin


Download now

Quick Protocol

RiboMAX Large Scale RNA Production Systems Quick Protocol FB059


[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

What’s in the box?

[wpdatatable id=327 table_view=regular]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

What’s in the box?

[wpdatatable id=329 table_view=regular]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB