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ReliaPrep™ RNA Miniprep Systems

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Description

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Size: 10 preps

Catalog number: Z6010

Size: 50 preps

Catalog number: Z6011

Size: 250 preps

Catalog number: Z6012

 

Size: 10 preps

Catalog number: Z6110

Size: 50 preps

Catalog number: Z6111

Size: 250 preps

Catalog number: Z6112

 

Recover Concentrated, Pure RNA from Precious Cell and Tissue Samples

Precious samples, sensitive assays, low copy messages – the effectiveness of RNA purification sets the stage for downstream results.

The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl).

Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.

  • Small sample size and low elution volume allows conservation of precious samples.
  • Maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets.
  • Usable, concentrated RNA is eluted in as little as 7µl. No need to further concentrate samples.
  • Rapid protocol and provided DNase reagents streamline laboratory processes.
  • Purified RNA is suitable for many common downstream assays.
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Higher RNA Yield

The data to the right show that the ReliaPrep™ Cell RNA Miniprep System efficiently isolates RNA from 100 to 1 x 106 HeLa cells with a higher RNA yield than the leading competitor.  Below, the RNA yield from 100,000 cells of 4 different cell lines. More data on performance of the ReliaPrep™ Systems is available in this Application Note.

 

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Yield and Purity of RNA from Tissues

This data shows typical RNA yields from fibrous and nonfibrous mouse tissues using the ReliaPrep™ RNA Tissue Miniprep System. RNA was isolated from 10mg samples of various mouse tissues. Yield and purity were determined by absorbance spectroscopy using a Nanodrop® 1000 instrument.

More data illustrating use of the ReliaPrep™ RNA Tissue Miniprep System is available in this Application Note.

Protocols

Complete Protocol

ReliaPrep™ RNA Cell Miniprep System Technical Manual


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ReliaPrep™ RNA Tissue Miniprep System Technical Manual

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Quick Protocols

ReliaPrep RNA Cell Miniprep System Quick Protocol FB131

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ReliaPrep RNA Tissue Miniprep System FB145

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What’s in the box?
Size: 10 preps

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?
Size: 50 preps

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[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?
Size: 250 preps

[wpdatatable id=210 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?
Size: 10 preps

[wpdatatable id=211 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?
Size: 50 preps

[wpdatatable id=212 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?
Size: 250 preps

[wpdatatable id=213 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions