Molecular Products Co.

Search
customer-support

Call Us Now:

0334-7752577

ReliaPrep™ FFPE Total RNA Miniprep System

Please Enquire

Description

[ts-heading h2=”Size” css_animation=”fadeInUp”][/ts-heading]

More Functional RNA Yield from FFPE Samples

The ability to extract RNA from FFPE samples allows correlation of disease state and tissue morphology with gene expression. Extraction of RNA from FFPE tissues is challenging because the formalin fixation process cross-links proteins and nucleic acids and introduces modifications that can inhibit subsequent analysis of RNA isolated from FFPE tissues.

The ReliaPrep™ FFPE Total RNA Miniprep System optimizes lysis conditions to reverse these RNA modifications without the need for overnight digestion. The system also uses a deparaffinization method that does not rely on xylene or other organic solvents. By eliminating the washing steps involved in xylene deparaffinization, this method can help retain small RNA fragments.

The protocol is complete in 1.5 hours with minimal hands-on time

[ts-heading h2=”Reliaprep™ FFPE Total RNA System Helps Preserve RNA Integrity” txt_align=”center” css_animation=”fadeInDown”]In this experiment, 5µl of purified RNA was used as template in a GoScript™ Reverse Transcription System reaction, and 5µl of the cDNA product was used as template for the multiplex PCR. Fragments from 80–290bp were detected.

[/ts-heading]

[ts-heading h2=”Simple Protocol” txt_align=”center” css_animation=”fadeInDown”][/ts-heading]
[ts-heading h2=”Customer Data” txt_align=”center” css_animation=”fadeInDown”][/ts-heading]

Sample Type: Rat kidney, 2 years old. Four 10µm FFPE sections were purified together (total amount = 40µm) and diluted in 40µl. Eight microliters were used for gel electrophoresis.

Sample Type: Rat kidney, 2 years old. Four 10µm FFPE sections were purified together (total amount = 40µm) and diluted in 40µl. Eight microliters were used for gel electrophoresis.

Sample Type: Human kidney tumor, 2 months old. Two samples were extracted using the total RNA kit. Total dilution volume was 30µl, and the loading volume for agarose gel electrophoresis was 15µl. Purity: A260 / A280 ~2.0 A260 / A230 ~2.1 Concentration: 200ng/µl.

Protocols

Complete Protocol

ReliaPrep™ FFPE Total RNA Miniprep System Technical Manual

 

Download Now

Quick Protocols

ReliaPrep FFPE RNA Miniprep System Quick Protocol FB125

 

Download Now

[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

What’s in the box?

[wpdatatable id=214 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=215 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions