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QuantiFluor® ssDNA System

Sensitive Quantitation of Single-Stranded DNA

  • More sensitive than NanoDrop for low-concentration samples
  • Requires less DNA than spectrophotometry

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Description

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Catalog number selected: E3190

Sensitive Quantitation of Single-Stranded DNA

 

Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ssDNA System contains a fluorescent dye that enables sensitive quantitation of small amounts of single-stranded (ssDNA) in solution. Detecting and quantitating ssDNA is useful many molecular biology research applications, including studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analysis of first-strand cDNAs, and quantitating bisulfate-treated DNA to study DNA methylation.

The QuantiFluor® ssDNA System requires less DNA (200pg) than other methods (~1 µg), so you save more of your sample for downstream assays.

Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems

For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.

The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent

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*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.

ssDNA Quantitation Sensitivity and Assay Linearity

The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

Protocols

Complete Protocol

QuantiFluor® ssDNA System Technical Manual

 

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Quick Protocols

QuantiFluor ssDNA System Quick Protocol FB198

 

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What’s in the box?

Item Part # Size
20X TE Buffer (pH 7.5) E260A 1 × 25ml
QuantiFluor® ssDNA Dye, 200X E284A 1 × 1ml
ssDNA Standard E288A 1 × 100μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.