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QuantiFluor® RNA System

Sensitive Quantitation of Small Amounts of RNA in Solution

  • Requires less template RNA than other systems
  • More sensitive than absorbance methods for low-concentration samples

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Description

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Catalog number selected: E3310

Sensitive Quantitation of Small Amounts of RNA in Solution

 

 

Highly Sensitive, Easy-to-Use, Fluorescent Dye for RNA Quantitation

The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is  important for many biological applications, including determining yield of in vitro transcribed RNA and  measuring RNA concentration before Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, RT-PCR and differential display PCR.

The QuantiFluor® System requires less RNA (100pg) than other RNA quantitation methods (~1µg), so you save more of your sample for downstream assays.

Improved Sensitivity vs. Absorbance and Other Fluorescent RNA Quantitation Systems

The system is designed to measure 0.1–500ng/µl RNA (from 1µl sample in a 200µl assay) and offers significantly increased sensitivity over absorbance at 260nm (NanoDrop) for samples containing low concentrations of RNA. Sensitivity is equivalent to that of Quant-iT™ RiboGreen®, with wider dynamic range.

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*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input RNA per assay.

RNA Quantitation Sensitivity and Assay Linearity

The QuantiFluor® RNA System detects as little as 0.5ng/ml RNA in a 96-well microplate assay (corresponding to 1µl of a 100ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

Protocols

Complete Protocol

QuantiFluor® RNA System

 

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Quick Protocols

QuantiFluor RNA System Quick Protocol FB199

 

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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.