Molecular Products Co.

Search
customer-support

Call Us Now:

0334-7752577

QuantiFluor® ONE dsDNA System

Fluorescent Quantitation of dsDNA with “Add-and-Read” Convenience

  • Specific quantitation of dsDNA
  • Increased sensitivity over absorbance at 260nm for low-concentration samples
  • Simple to use and automation-friendly

Please Enquire

Description

[ts-heading h2=”Size” css_animation=”fadeInUp”][/ts-heading]

Catalog number selected: E4871

Catalog number selected: E4870

Sensitive Quantitation of Double-Stranded DNA in Solution

Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nm Ex/531nm Em) in an automation-friendly, “Add-and-Read” format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It’s as easy to use as NanoDrop® absorbance-based methods, but much more sensitive for low-concentration samples.

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR.  QuantiFluor® ONE dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).

[wpdatatable id=137 table_view=regular]

*Based on 1µl sample input per assay.

DNA Quantitation Assay Sensitivity and Linearity

The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).

How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

Protocols

Complete Protocol

QuantiFluor® ONE dsDNA System

 

Dwonload Now

Quick Protocols

QuantiFluor ONE dsDNA System Quick Protocol FB200

 

Dwonload Now

[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

What’s in the box?

Item Part # Size
QuantiFluor® ONE dsDNA Dye E489A 1 × 20ml
QuantiFluor® ONE Lambda DNA E490B 1 × 80μg
1X TE Buffer (pH 7.5) E497B 1 × 10ml
[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

Item Part # Size
QuantiFluor® ONE dsDNA Dye E489A 5 × 20ml
QuantiFluor® ONE Lambda DNA E490A 1 × 400μg
1X TE Buffer (pH 7.5) E497A 1 × 25ml
[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.