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qPCR Kits: GoTaq® qPCR and RT-qPCR Systems

  • Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Please Enquire

Description

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Size:

5ml : Catalog number selected:  A6001

25ml: Catalog number selected: A6002

 

Size:

5ml : Catalog number selected: A6020

 

Size:

5ml : Catalog number selected: A6010

Simple, Sensitive, Dye-Based qPCR and RT-qPCR

The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a  fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.

Rapid hot-start activation and processive enzymes make the  GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.

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Sensitivity and Specificity for qPCR

The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.

Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:

  • Sensitive detection for earlier quantitation of low- and high-copy targets.
  • Room-temperature setup,making it suitable for automation and high-throughput detection.
  • Compatibility with both fast and standard qPCR cycling methods.
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Size Cat.# 10µl Reactions 20µl Reactions
A6001 5ml 1,000 reactions 500 reactions
A6002 25ml 5,000 reactions 2,500 reactions

qPCR with single-copy detection. The GAPDH gene was amplified and detected from 10-fold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (Slope=-3.2; R2=0.995). Kit yield and purity were measured by absorbance spectroscopy.

Reverse Transcription and qPCR in One Reaction

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Other features provided by the 1-Step System include:

  • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
  • Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
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Size Cat.# 10µl Reactions 20µl Reactions
A6020 5ml 1,000 reactions 500 reactions

Linear dynamic range from 100ng down to 10fg of human RNA template. Seven 10-fold dilutions of human total RNA was run using 200nM of each GAPDH transcript specific primer. The amplification curve shows 97% efficiency and an r2=0.999.

Two-Step Reverse Transcription and qPCR

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

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Size Cat.# 10µl Reactions 20µl Reactions
A6010 5ml 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions 50 × 20µl RT reactions and 500 × 20µl qPCR reactions

Extended linear range. Dilutions of human reference total RNA were amplified with GoTaq® 2-Step RT-qPCR System looking at 5.8S expression. GoTaq® 2-Step system demonstrates a linear dynamic range over 9 orders of  magnitude with a high fluorescent signal. NTC reactions were all blank.

Suitable for Different Organisms and Sample Types

DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

Organism Types:

  • Animal
  • Bacteria
  • Fungi
  • Human
  • Parasites
  • Viruses
  • Yeast

Sample Types:

  • Blood
  • Cells
  • Feces
  • FFPE
  • Food
  • Human Cerebrospinal Fluid (CSF)
  • Plants
  • Plasma
  • Saliva
  • Serum
  • Sputum
  • Swabs
  • Tissue
  • Urine

Compatible with these Instruments

For further details on instrument compatibility, please reference the appropriate Technical Manual. Instruments not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

  • Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System
  • Applied Biosystems 7300 and 7900HT Real-Time PCR System
  • Applied Biosystems GeneAmp® 5700 Thermal Cycler
  • Applied Biosystems StepOne™ and StepOnePlus™ Real-Time PCR Systems
  • Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
  • Applied Biosystems ViiA® 7 Real-Time PCR System
  • Applied Biosystems QuantStudio® Real Time PCR Systems
  • Bio-Rad CFX96 Real-Time PCR Detection System
  • Bio-Rad DNA Engine Opticon® and Opticon® 2 Real Time PCR Detection Systems
  • Bio-Rad iCycler® iQ™ and iQ™ 5 Real-Time PCR Detection System
  • Bio-Rad/MJ Research Chromo4™ Real-Time Detector
  • Bio-Rad MyiQ™ Real-Time PCR Detection System
  • Cepheid SmartCycler® System
  • Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer
  • Eppendorf Mastercycler® ep realplex Real-Time PCR System
  • Roche LightCycler® 480 Real-Time PCR System
  • Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems
  • Stratagene Mx4000® Multiplex Quantitative PCR System
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Complete Protocol

GoTaq® qPCR Master Mix Technical Manual

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GoTaq® 1-Step RT-qPCR System Technical Manual
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-1-Step-RTqPCR-System-TM355.zip” text=”Download” color=”blue_three” ]

GoTaq® 2-Step RT-qPCR System Technical Manual
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-2-Step-RT-qPCR-System-TM337.zip” text=”Download” color=”blue_three” ]

Quick Protocols

 

GoTaq qPCR Master Mix Quick Protocol FB103

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-qPCR-Master-Mix-Quick-Protocol-FB103.zip” text=”Download” color=”blue_three” ]


GoTaq OneStep RT qPCR System Quick Protocol FB127
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-OneStep-RT-qPCR-System-Quick-Protocol-FB127.zip” text=”Download” color=”blue_three” ]

 

GoTaq® 2-Step RT-qPCR System Quick Protocol FB115
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-2-Step-RT-qPCR-System-Quick-Protocol-FB115.zip” text=”Download” color=”blue_three” ]
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What’s in the box?

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SDS For  A6001
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A6001.zip” text=”Download SDS” color=”blue_three” ]

SDS For A6002
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A6002.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

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SDS For  A6020
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A6020.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=77 table_view=regular]

SDS For A6010
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A6010.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions