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PvuI

  • Active and capable of digestion directly in GoTaq® Green Master Mix

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Description

2-10μ/μl

Catalog number selected: R6321

100u

Catalog number selected: R6321

500u

Catalog number selected: R6325

Cut Site:

CG   ATCG
GCTA   GC

Usage Notes:

  1. Religation of DNA fragments with dam methylation is difficult.
  2. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Incubation Conditions: Buffer D. 37°C.

Source: Proteus vulgaris.

Storage Buffer: 10mM Tris-HCl (pH 7.3 @ 25°C), 50mM NaCl, 0.1% Triton® X-100, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

You are viewing: R6321 Change Configuration

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6325 Change Configuration

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SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions